Molecular characterization and interactome analysis of Trypanosoma cruzi tryparedoxin II

Journal of Proteomics
Diego G AriasCarlos Robello

Abstract

Trypanosoma cruzi, the causative agent of Chagas disease, possesses two tryparedoxins (TcTXNI and TcTXNII), belonging to the thioredoxin superfamily. TXNs are oxidoreductases which mediate electron transfer between trypanothione and peroxiredoxins. This constitutes a difference with the host cells, in which these activities are mediated by thioredoxins. These differences make TXNs an attractive target for drug development. In a previous work we characterized TcTXNI, including the redox interactome. In this work we extend the study to TcTXNII. We demonstrate that TcTXNII is a transmembrane protein anchored to the surface of the mitochondria and endoplasmic reticulum, with a cytoplasmatic orientation of the redox domain. It would be expressed during the metacyclogenesis process. In order to continue with the characterization of the redox interactome of T. cruzi, we designed an active site mutant TcTXNII lacking the resolving cysteine, and through the expression of this mutant protein and incubation with T. cruzi proteins, heterodisulfide complexes were isolated by affinity chromatography and identified by mass spectrometry. This allowed us to identify sixteen TcTXNII interacting proteins, which are involved in a wide range of cel...Continue Reading

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Citations

Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Alice Machado-SilvaCarlos Renato Machado
Feb 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alberto Guevara-FloresIrene Patricia Del Arenal
Apr 11, 2018·Antioxidants & Redox Signaling·David YoungJoris Messens
Feb 23, 2019·The Journal of Experimental Medicine·Lucía PiacenzaRafael Radi
Sep 28, 2018·Current Medicinal Chemistry·Alan TaleviMarcelo Comini
Jan 11, 2020·Frontiers in Cellular and Infection Microbiology·Andrea C MesíasM Paola Zago
Jan 23, 2021·RSC Medicinal Chemistry·Anutthaman Parthasarathy, Karunakaran Kalesh
Jul 19, 2020·Free Radical Biology & Medicine·Diego G AriasSergio A Guerrero

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