Molecular characterization and localization of the RIC-3 protein, an effector of nicotinic acetylcholine receptor expression

Journal of Neurochemistry
Francisco CastelánManuel Criado

Abstract

The RIC-3 protein acts as a regulator of acetylcholine nicotinic receptor (nAChR) expression. In Xenopus laevis oocytes the human RIC-3 (hRIC-3) protein enhances expression of alpha7 receptors and abolishes expression of alpha4beta2 receptors. In vitro translation of hRIC-3 evidenced its membrane insertion but not the role as signal peptide of its first transmembrane domain (TMD). When the TMDs of hRIC-3 were substituted, its effects on nAChR expression were attenuated. A certain linker length between the TMDs was also needed for alpha7 expression enhancement but not for alpha4beta2 inhibition. A combination of increased alpha7 receptor steady state levels, facilitated transport and reduced receptor internalization appears to be responsible for the increase in alpha7 membrane expression induced by hRIC-3. Antibodies against hRIC-3 showed its expression in SH-SY5Y and PC12 cells and its induction upon differentiation. Immunohistochemistry demonstrated the presence of RIC-3 in rat brain localized, in general, in places where alpha7 nAChRs were found.

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Citations

Jan 29, 2011·The Journal of Pharmacology and Experimental Therapeutics·Manickavasagom AlkondonEdson X Albuquerque
Oct 9, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ying WangZuo-Zhong Wang
Jul 30, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John K AlexanderWilliam N Green
Aug 21, 2009·Journal of Molecular Neuroscience : MN·Khosrow RezvaniMariella De Biasi
Feb 5, 2008·British Journal of Pharmacology·N S Millar
Mar 17, 2016·Cellular and Molecular Life Sciences : CMLS·Hans O Kalkman, Dominik Feuerbach
Apr 12, 2016·Frontiers in Pharmacology·Lieven D DeclercqGuy Bormans
Feb 24, 2015·Neuropharmacology·Melissa L SinkusSherry Leonard
Mar 17, 2015·European Journal of Pharmacology·Saeed Sadigh-EteghadShirin Babri
Nov 2, 2011·Biochimica Et Biophysica Acta·Ana S Vallés, Francisco J Barrantes

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