Molecular characterization and nonradioactive detection of beta-thalassemia in Malaysia

Acta Haematologica
S FucharoenY Fukumaki

Abstract

The spectrum of beta-thalassemia mutations in Malaysia has been determined in 45 beta-thalassemia chromosomes using dot blot hybridization of the polymerase chain reaction amplified DNA and direct DNA sequencing. Eleven different molecular defects, including those previously detected in Chinese, Asian Indians, and American blacks, and a novel frameshift mutation causing beta zero-thalassemia were detected. Since this novel mutation, a T deletion in codon 15 creates a new restriction site for EcoRII enzyme; the mutation could be detected by EcoRII digestion of the appropriate amplified fragment. The results of the present study provide additional information on the molecular heterogeneity of beta-thalassemia in this population. We also demonstrated the nonradioactive detection method of the beta-thalassemia mutation based upon the digoxigenin-labeled oligonucleotide probes.

Citations

Jun 30, 1994·Journal of Biotechnology·C Kessler
Jun 13, 2002·British Journal of Haematology·Ne WinAye-Aye Myint
Oct 1, 2008·Respiratory Research·Tricia M McKeeverJohn Britton
Oct 1, 1993·Hemoglobin·A MeratM H Steinberg
Oct 1, 1991·American Journal of Hematology·S FucharoenY Fukumaki
Feb 3, 2004·American Journal of Hematology·Bani GajraGeeta Talukder
May 3, 2019·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Pornswan WasantPoh-San Lai
Nov 1, 1992·American Journal of Hematology·V LaosombatY Fukumaki

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