PMID: 8590276Jan 1, 1995Paper

Molecular characterization and ocular hypotensive properties of the prostanoid EP2 receptor

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
David F WoodwardS W Andrews

Abstract

The cloning of the genes that encode for prostaglandin (PG) receptors has resolved much of the complexity and controversy in this area by confirming the classification proposed by Coleman, et al. Two issues that remained unresolved were (1) the inability of the EP2 agonist butaprost to interact with the cloned putative EP2 receptor and (2) molecular biological confirmation of a fourth PGE2-sensitive receptor, which was pharmacologically designated EP4. In order to provide clarification, we attempted to clone further PGE2-sensitive receptors. By using a cDNA probe that encodes for the human EP3A receptor, a cDNA clone that encoded for a novel PGE2-sensitive receptor was obtained by screening a human placenta library. This cDNA clone was transfected into COS-7 cells for pharmacological studies. The cDNA clone obtained from human placenta had only about 30% amino acid identity with cDNAs for other PG receptors, including those that encode for the previously proposed murine and human EP2 receptors. Radioligand binding studies on the novel EP receptor expressed in COS-7 cells revealed that selective EP2 agonists such as butaprost, AH 13205, AY 23626 and 19(R)-OH PGE2 all competed with 3H-PGE2 for its binding sites, whereas selective...Continue Reading

References

Jan 1, 1991·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·M NakajimaO Hayaishi
Jan 1, 1990·Annals of the New York Academy of Sciences·W Aretz, K Sauber
Nov 1, 1989·The Biochemical Journal·S MuallemD T Yamaguchi
Jun 1, 1980·Prostaglandins·P J Gardiner, H O Collier

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Citations

Apr 27, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·J Y Crider, N A Sharif
Feb 1, 1997·Survey of Ophthalmology·D F WoodwardA Ocklind
Jul 6, 2010·Prostaglandins & Other Lipid Mediators·Cui YangGeorge Pak-Heng Leung
Nov 18, 2005·Advanced Drug Delivery Reviews·Patrick M HughesClive G Wilson
Sep 24, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Sumana BiswasChristopher A Paterson
Jul 23, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·David F WoodwardCarol B Toris

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