Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China.

Virus Genes
Jian-Fei ChenLi Feng

Abstract

Six porcine epidemic diarrhea viruses (PEDVs) were isolated from the fecal samples of piglets infected with PEDV in 2006 in China. The membrane (M) protein genes of six PEDV isolates were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR), then cloned, sequenced, and compared with each other as well as those ten PEDV reference strains. The M protein genes of six Chinese PEDV isolates consisted of 692 nucleotides containing a single open reading frame (ORF) of 681 nucleotides, which encoded a 226aa-long peptide. The conserved intergenic motif (ATAAAC), as previously recognized in Br1/87, was found in the 5 nucleotides upstream of the initiator ATG of M protein genes of six Chinese PEDV isolates. The hexamer motif was also found in CV777, JMe2, LZC, and QH. The M protein of six isolates had three main transmembrane domains (aa20-38, aa43-65, aa75-97). The M protein of one isolate, CH/IMT/06, had one potential glycosylation site, but those of the other five isolates had two. The glycosylation sequence Asn-Phe-Thr was highly conserved in the M proteins of six PEDV isolates. The six PEDV isolates showed nucleotide sequence homology between 98.8 and 100% and deduced amino acid sequence homology between 98.2 and 100...Continue Reading

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Citations

Jun 15, 2010·Archives of Virology·Jianfei ChenLi Feng
Oct 12, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Liyan CaoXiaofeng Ren
Jul 25, 2009·Emerging Infectious Diseases·Suphasawatt PuranavejaRoongroje Thanawongnuwech
Jun 25, 2014·Emerging Infectious Diseases·Sunhee Lee, Changhee Lee
Mar 1, 2012·Transboundary and Emerging Diseases·S Nuntawan Na AyudhyaR Thanawongnuwech
Jun 1, 2015·Archives of Virology·Dam Thi VuiDachrit Nilubol
Aug 15, 2015·Clinical and Experimental Vaccine Research·Daesub SongBokyu Kang
Sep 23, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ye ChenChong Wang
May 23, 2009·Pharmacology & Therapeutics·Karen E Porter, Neil A Turner
Feb 14, 2018·Veterinary Sciences·Yonghyan KimMaxim C-J Cheeran
Oct 23, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Jinliang WangZhiqiang Shen
Feb 7, 2019·Archives of Virology·Mariia NefedevaAlexander Malogolovkin
Sep 8, 2018·Tropical Animal Health and Production·Xing GaoJiakui Li
Oct 31, 2014·EFSA Journal·UNKNOWN EFSA Panel on Animal Health and Welfare (AHAW)
Nov 6, 2015·The Journal of Veterinary Medical Science·Dongbo SunLi Feng
Jul 28, 2015·Microbes and Environments·Hyeon-Woo KohSoo-Je Park

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Datasets Mentioned

BETA
EU033964
EU033962
EU033965
EU033967
EU033966
AF015888
AF353511
AF019893
AY608890
D89752

Methods Mentioned

BETA
PCR
electrophoresis
M13 sequencing
glycosylation

Software Mentioned

TMHMM
MegAlign
NetNGlyc
Lasergene
DNAMAN

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