Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea viruses associated with outbreaks of severe diarrhea in piglets in Jiangxi, China 2013

PloS One
Deping SongYuxin Tang

Abstract

Porcine epidemic diarrhea (PED), caused by porcine epidemic diarrhea virus (PEDV), is a highly contagious, acute enteric viral disease of swine characterized by vomiting, watery diarrhea, dehydration and death. To identify and characterize the field PEDVs associated with the outbreaks of severe diarrhea in piglets in Jiangxi, 2013, the complete genome sequences of two representative strains of PEDV, designated CH/JX-1/2013 and CH/JX-2/2013, were determined and analyzed. The genome sequences of both emergent Jiangxi PEDV strains, CH/JX-1/2013 and CH/JX-2/2013, were 28,038 nucleotides in length excluding 3' poly (A) tail. Compared to the PEDV CV777 strain, CH/JX-1/2013 and CH/JX-2/2013 had some unique genetic characteristics in the proximal region of the 5´-UTRs. Phylogenetic analysis of the complete genomes and the structural proteins revealed that CH/JX-1/2013 and CH/JX-2/2013 had a close relationship with post-2010 Chinese PEDV strains and US strains identified in 2013. The nucleotide identity between the two Jiangxi strains (CH/JX-1/2013 and CH/JX-2/2013) and 30 strains of PEDV identified ante-2010 and post-2010 ranged from 96.3-97.0% and 97.3-99.7%, respectively. Multiple nucleotide and deduced amino acid mutations were obse...Continue Reading

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Citations

Nov 22, 2015·Preventive Veterinary Medicine·Yosuke SasakiAndres Perez
Jun 1, 2015·Archives of Virology·Dam Thi VuiDachrit Nilubol
Jul 1, 2016·BMC Veterinary Research·María Elena Trujillo-OrtegaRosa Elena Sarmiento-Silva
Dec 16, 2017·Virus Genes·Rocío Lara-RomeroJosé Francisco Rivera-Benítez
Feb 17, 2016·EFSA Journal·UNKNOWN European Food Safety Authority (EFSA)
Sep 23, 2020·Animals : an Open Access Journal From MDPI·Yuhan ZhangYuxin Tang

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Datasets Mentioned

BETA
KF760557
GU937797

Software Mentioned

Protean
SeqMan
DNAStar Lasergene
Primer
MEGA

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