Molecular Characterization and Potential Synthetic Applications of GH1 β-Glucosidase from Higher Termite Microcerotermes annandalei

Applied Biochemistry and Biotechnology
Siriphan ArthornthurasukPrachumporn T Kongsaeree

Abstract

A novel β-glucosidase from higher termite Microcerotermes annandalei (MaBG) was obtained via a screening method targeting β-glucosidases with increased activities in the presence of glucose. The purified natural MaBG showed a subunit molecular weight of 55 kDa and existed in a native form as a dimer without any glycosylation. Gene-specific primers designed from its partial amino acid sequences were used to amplify the corresponding 1,419-bp coding sequence of MaBG which encodes a 472-amino acid glycoside hydrolase family 1 (GH1) β-glucosidase. When expressed in Komagataella pastoris, the recombinant MaBG appeared as a ~ 55-kDa protein without glycosylation modifications. Kinetic parameters as well as the lack of secretion signal suggested that MaBG is an intracellular enzyme and not involved in cellulolysis. The hydrolytic activities of MaBG were enhanced in the presence of up to 3.5-4.5 M glucose, partly due to its strong transglucosylation activity, which suggests its applicability in biosynthetic processes. The potential synthetic activities of the recombinant MaBG were demonstrated in the synthesis of para-nitrophenyl-β-D-gentiobioside via transglucosylation and octyl glucoside via reverse hydrolysis. The information obtain...Continue Reading

References

Jun 1, 1997·Bioscience, Biotechnology, and Biochemistry·T R Yan, C L Lin
Dec 19, 2002·Critical Reviews in Biotechnology·Yukti BhatiaV S Bisaria
Mar 27, 2003·Applied Microbiology and Biotechnology·M Ohkuma
May 24, 2003·Biochemical and Biophysical Research Communications·Jisnuson SvastiRakrudee Sarnthima
Nov 4, 2004·FEMS Microbiology Letters·Fabiana Fonseca ZanoeloJoão Atílio Jorge
Oct 13, 2005·The FEBS Journal·Stephen F AltschulYi-Kuo Yu
Nov 14, 2007·Archives of Biochemistry and Biophysics·Phimonphan ChuankhayanJames R Ketudat Cairns
Mar 14, 2008·Journal of Industrial Microbiology & Biotechnology·Raj KumarOm V Singh
Aug 12, 2009·Applied Biochemistry and Biotechnology·Bruno BenolielLidia Maria Pepe de Moraes
Jan 14, 2010·Journal of Biomedicine & Biotechnology·Chantragan SrisomsapJisnuson Svasti
May 22, 2010·Cellular and Molecular Life Sciences : CMLS·James R Ketudat Cairns, Asim Esen
Oct 5, 2010·Journal of Microbiology and Biotechnology·Zemin FangYazhong Xiao
Dec 3, 2010·Cold Spring Harbor Perspectives in Biology·R Martin VabulasF Ulrich Hartl
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Dec 8, 2011·Applied Microbiology and Biotechnology·Dina JabbourGarabed Antranikian
Mar 16, 2012·Carbohydrate Research·Salila PengthaisongJames R Ketudat Cairns
May 31, 2012·Lipids in Health and Disease·Etsuko MurakiKeizo Kasono
Oct 9, 2012·Enzyme and Microbial Technology·Qianfu WangZhihua Zhou
Oct 17, 2012·Bioresource Technology·Reeta Rani SinghaniaAshok Pandey
Apr 16, 2013·The EMBO Journal·Catharina SteentoftHenrik Clausen
Jun 14, 2013·The Journal of General and Applied Microbiology·Cristiane Akemi UchimaManabu Arioka
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 3, 2014·Archives of Insect Biochemistry and Physiology·Hatibe Ertürk KaraSelma Sinan
Sep 18, 2014·Bioscience, Biotechnology, and Biochemistry·Preeyanuch ThongpooPrachumporn T Kongsaeree

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