Molecular characterization and transcription regulation analysis of type I IFN gene in grass carp (Ctenopharyngodon idella)

Gene
Dongming LiCheng-Yu Hu

Abstract

Type I interferons and interferon regulatory factor 7 (IRF7), which are crucial for innate immunity against viral infection, have been identified in many teleost fishes in recent years. In this study, the complete genomic sequence of grass carp (Ctenopharyngodon idella) type I interferon (termed CiIFN) (GU139255) and the full-length IRF7 cDNA sequence of grass carp (termed CiIRF7) (GQ141741) were cloned and characterized. CiIFN consists of 3368 bp, retaining the characteristic 5-exon/4-intron gene organization in fish type I IFNs. The CiIFN spans 5 exons and encodes a polypeptide of 180 amino acids, with the first 22 amino acids representing a putative signal peptide. The CiIFN promoter sequence was found to be 760 bp, which can be divided into a proximal region (from -1 to -140 bp) and a distal region (from -400 to -700 bp). The cDNA of CiIRF7 was found to be 1808 bp in full length, with an ORF of 1293 bp that encodes a putative protein of 430 amino acids. The putative amino acid sequence of CiIRF7 possesses a DNA-binding domain (DBD) in the N-terminal region. Real-time PCR analysis revealed that CiIFN displayed a low constitutive expression in all the tissues tested. After stimulation by polyinosinic:polycytidylic acid (Poly ...Continue Reading

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Citations

Oct 3, 2013·Fish & Shellfish Immunology·You-Sheng HuCheng-Yu Hu
Jul 18, 2015·Biology·Sarah PoynterStephanie DeWitte-Orr
Jun 9, 2015·Fish & Shellfish Immunology·Kittipong ThanasaksiriHidehiro Kondo
May 5, 2016·Fish & Shellfish Immunology·Haizhou WangChengyu Hu
Aug 23, 2019·Developmental and Comparative Immunology·Yinping LiChengyu Hu

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