Molecular characterization, genomic arrangement, and expression of bmpD, a new member of the bmp class of genes encoding membrane proteins of Borrelia burgdorferi.

Infection and Immunity
R RamamoorthyT Philipp M

Abstract

An expression library made with Borrelia burgdorferi DNA in the vector lambda ZapII was screened with serum from a monkey infected with the Lyme disease agent. This serum killed B. burgdorferi in vitro by an antibody-dependent, complement-mediated mechanism and contained antibodies to at least seven spirochetal antigens, none of which were the major outer surface proteins OspA or OspB. Among several positive clones, a clone containing the B. burgdorferi bmpA gene encoding the immunodominant antigen P39 was obtained. Chromosome walking and DNA sequence analysis permitted the identification of two additional upstream genes homologous to the bmpA gene and its related companion, bmpB. The first of these was the recently characterized bmpC gene, and adjacent to it was the fourth and new member of this class, which has been designated bmpD. The gene product encoded by bmpD is 34l residues long, contains a signal sequence with a potential signal peptidase II cleavage site, and has 26% identity with TmpC of Treponema pallidum. Southern blotting confirmed the tandem arrangement of all four bmp genes in the chromosome of B. burgdorferi JD1. However, Northern (RNA) blotting revealed that bmpD is expressed as a monocistronic transcript, wh...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 1, 1990·Infection and Immunity·M A SwancuttM V Norgard
Mar 1, 1987·Journal of Clinical Microbiology·J PiesmanA Spielman
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A von GabainS N Cohen
Jun 1, 1995·Journal of Bacteriology·M TheisenK Hansen
Nov 1, 1994·Trends in Microbiology·M T Philipp, B J Johnson
Jun 1, 1993·Infection and Immunity·R T MarconiC F Garon
May 1, 1993·Journal of Clinical Microbiology·R J PollackA Spielman

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Citations

Dec 22, 2005·Infection and Immunity·Chad S BrooksDarrin R Akins
Mar 14, 2000·Journal of Bacteriology·V Y GorbachevaF C Cabello
Jul 16, 2005·Clinical Microbiology Reviews·Maria E Aguero-RosenfeldGary P Wormser
Jul 18, 2002·The Journal of Experimental Medicine·Fang Ting LiangErol Fikrig
Sep 6, 2007·Molecular Microbiology·Styliani AntonaraJenifer Coburn
Dec 21, 2013·PloS One·Linxu ChenFang Ting Liang
Aug 26, 2009·Infection and Immunity·Ashutosh VermaBrian Stevenson
Jan 1, 1997·Annual Review of Immunology·L H Sigal
Apr 5, 2005·Journal of Bacteriology·Ramesh RamamoorthyDorothy Scholl-Meeker
Oct 24, 1998·Infection and Immunity·R Ramamoorthy, M T Philipp
Apr 1, 1997·Infection and Immunity·K J IndestM T Philipp
May 3, 2008·Infectious Disease Clinics of North America·Maria E Aguero-Rosenfeld

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