Molecular characterization, genomic structure and expressional profiles of a CXC chemokine receptor 4 (CXCR4) from rock bream Oplegnathus fasciatus

Fish & Shellfish Immunology
William Shanthakumar ThulasithaJehee Lee

Abstract

The CXC chemokine receptor 4 (CXCR4) is the cognate receptor of the CXC chemokine ligand 12 (CXCL12) and plays a pivotal role under immune-pathophysiological conditions. In the current study, the CXCR4 homolog of Oplegnathus fasciatus (OfCXCR4) was sequenced and the mRNA expression levels were characterized. The genomic structure of the cloned OfCXCR4 coding region (2094 bp) revealed a bi-exonic element, where the open reading frame (ORF) appears split by a single intron. Analysis of the ORF (1134 bp) of OfCXCR4 revealed a predicted protein of 42.1 kDa with typical seven transmembrane (TM) domain architecture and several conserved structural features, including two cysteine residues forming a predicted disulfide bond, a characteristic CXC motif (containing CYC) and a G-protein-coupled receptor (GPCR) family 1 signature. Furthermore, based on comparative analysis, the structure OfCXCR4 appears well conserved at both the genomic DNA and the amino acid levels. Phylogenic analysis of OfCXCR4 revealed that the greatest homology was with its teleostean relatives. Expression studies showed ubiquitous OfCXCR4 transcription, mainly in immune organs, with the highest levels in the head kidney. Examination of OfCXCR4 transcriptional regul...Continue Reading

References

Jan 1, 1992·DNA and Cell Biology·W C ProbstS C Sealfon
May 23, 1998·FEBS Letters·A CaruzF Arenzana-Seisdedos
Jul 25, 2000·Developmental and Comparative Immunology·B Y AlabyevA V Taranin
Oct 20, 2001·The Journal of Biological Chemistry·A Marchese, J L Benovic
Dec 4, 2001·Mechanisms of Development·S W ChongV Korzh
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 1, 2003·Nature·Holger KnautUNKNOWN Tübingen 2000 Screen Consortium
Nov 5, 2003·The Journal of Experimental Medicine·Tiziana SchioppaAntonio Sica
Jul 6, 2004·Nature Medicine·Daniel J CeradiniGeoffrey C Gurtner
Jul 26, 2006·Experimental Hematology·Carol SheridanHarikrishna Nakshatri
Feb 24, 2007·Veterinary Immunology and Immunopathology·Shuichi TsuchidaToshikazu Takahashi
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hiromi KurokawaMinoru Tanaka
Jan 12, 2008·Biochemical and Biophysical Research Communications·Hisaki Hayashi, Tsutomu Kume
Sep 15, 2009·Journal of Biomedicine & Biotechnology·Airong Jia, Xiao-Hua Zhang
Jan 14, 2010·Molecular Cancer Research : MCR·Maciej TarnowskiMariusz Z Ratajczak
Jul 3, 2010·Pathology International·Bungo FurusatoJohng S Rhim
Mar 19, 2011·Developmental and Comparative Immunology·Alí Alejo, Carolina Tafalla
Jul 6, 2011·Developmental and Comparative Immunology·Ching-Yu LinTzong-Yueh Chen
May 25, 2013·Developmental and Comparative Immunology·Jun ChenJun Zou
Jan 15, 2015·General and Comparative Endocrinology·Ten-Tsao Wong, Yonathan Zohar

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