Molecular characterization of a 35-kilodalton protein of Borrelia burgdorferi, an antigen of diagnostic importance in early Lyme disease.

Journal of Clinical Microbiology
R D GilmoreB J Johnson

Abstract

Antibodies against a 35-kDa antigen of Borrelia burgdorferi are detectable in the serum of about half of patients with early Lyme disease. The gene encoding this antigen was isolated from a genomic library of B. burgdorferi B31 (low passage), and full-length expression of the recombinant gene product was achieved in Escherichia coli. Antiserum raised against the recombinant protein was reactive with a B. burgdorferi protein of the same molecular size as the diagnostic 35-kDa antigen cited in an earlier study of criteria for the sero-diagnosis of early Lyme disease. Also, the recombinant protein was reactive with serum from patients with early Lyme disease who were seropositive for the 35-kDa antigen. DNA sequence analysis of the gene indicated an open reading frame of 909 bp encoding a protein with a calculated molecular mass of 34.3 kDa. This gene did not possess the usual initiation codon ATG but rather probably used a TTG codon. The deduced amino acid sequence of the N terminus exhibited a motif similar to that for signal peptides of lipoproteins. Southern blotting revealed a chromosomal location for this gene; and it was specific for B. burgdorferi, B. afzellii, and B. garinii but not for B. hermsii, B. coriaciae, or B. tur...Continue Reading

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Citations

Jun 5, 1998·Annual Review of Public Health·D H Walker
Feb 26, 2000·Infection and Immunity·J AnguitaE Fikrig
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert D GilmoreJames A Carroll
May 1, 2012·FEMS Immunology and Medical Microbiology·Melisha R KenedyDarrin R Akins
Feb 7, 2014·Clinical and Vaccine Immunology : CVI·Kevin S BrandtRobert D Gilmore
Mar 20, 2001·Infection and Immunity·R Ramamoorthy, D Scholl-Meeker
Nov 7, 2007·Infection and Immunity·Jill A LivengoodRobert D Gilmore
Jun 23, 2006·Infection and Immunity·Andrew J NowalkJames A Carroll
Oct 24, 1998·Infection and Immunity·R Ramamoorthy, M T Philipp
Apr 1, 1997·Infection and Immunity·K J IndestM T Philipp

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