PMID: 8596666Oct 1, 1995Paper

Molecular characterization of a 40-kDa outer membrane protein, FomA, of Fusobacterium periodonticum and comparison with Fusobacterium nucleatum

Oral Microbiology and Immunology
A I BolstadH B Jensen

Abstract

The 40 kDa-outer membrane protein FomA of Fusobacterium periodonticum ATCC 33693 was found to exhibit heat modifiable properties, typical for a porin, and N-terminal sequencing indicated a close relationship to the porin FomA of Fusobacterium nucleatum. A polymerase chain reaction approach was therefore applied for sequencing the fomA gene of F. periodonticum, and nucleotide and deduced amino acid sequences were aligned and compared with the corresponding sequences of different strains of F. nucleatum. In all strains we found a common protein upstream of the fomA gene. The noncoding area upstream of the putative -35 region of the F. periodonticum fomA gene exhibited little sequence similarity with the F. nucleatum gene. The transcriptional unit of FomA, on the other hand, was very similar, with the similarities concentrated in domains that were interspersed with hypervariable regions. A topology model was made and compared with those made for F. nucleatum. This indicated that the great similarities reside in the membrane-spanning segments of the protein, while most cell surface exposed loops were hypervariable. The results strongly support the proposed model for FomA and also indicate that these taxa are related but on a lower ...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Jan 1, 1978·Annual Review of Microbiology·M Riley, A Anilionis
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 25, 1991·FEBS Letters·M S WeissG E Schulz
Nov 1, 1991·Journal of Clinical Periodontology·W E MooreH A Schenkein
Sep 1, 1991·Journal of Periodontal Research·J M GoodsonS M Watanabe
Feb 1, 1989·Scandinavian Journal of Dental Research·V BakkenH B Jensen
Jan 1, 1984·Annual Review of Biochemistry·P H von HippelJ A McSwiggen
Jun 1, 1983·Journal of Dental Research·T V PottsJ Slots
Sep 1, 1983·Journal of Dental Research·J SlotsP A Mashimo
Dec 1, 1983·Journal of Dental Research·D A CalhoonJ Slots
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 22, 1994·Journal of Molecular Biology·A KarshikoffT Schirmer
Apr 1, 1994·International Journal of Systematic Bacteriology·L A NicholsonA L Hodgson
Mar 1, 1993·Journal of Clinical Microbiology·A I Bolstad, H B Jensen
Jun 1, 1994·Periodontology 2000·A TannerF E Dewhirst

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Citations

Jul 21, 2005·Journal of Bacteriology·Yiping W HanCheri X Deng
Jan 1, 1996·Clinical Microbiology Reviews·A I BolstadV Bakken

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