PMID: 9431678Jan 1, 1997Paper

Molecular characterization of a bean alpha-amylase inhibitor that inhibits the alpha-amylase of the mexican bean weevil Zabrotes subfasciatus

Planta
M F Grossi de SaM J Chrispeels

Abstract

Cultivated varieties of the common bean (Phaseolus vulgaris L.) contain an alpha-amylase inhibitor (alpha AI-1) that inhibits porcine pancreatic alpha-amylase (PPA; EC 3.2.1.1) and the amylases of certain seed weevils, but not that of the Mexican bean weevil, Zabrotes subfasciatus. A variant of alpha AI-1, called alpha AI-2, is found in certain arcelin-containing wild accessions of the common bean. The variant alpha AI-2 inhibits Z. subfasciatus alpha-amylase (ZSA), but not PPA. We purified alpha AI-2 and studied its interaction with ZSA. The formation of the alpha AI-2-ZSA complex is time-dependent and occurs maximally at pH 5.0 or below. When a previously isolated cDNA assumed to encode alpha AI-2 was expressed in transgenic tobacco seeds, the seeds contained inhibitory activity toward ZSA but not toward PPA, confirming that the cDNA encodes alpha AI-2. The inhibitors alpha AI-1 and alpha AI-2 share 78% sequence identity at the amino acid level and they differ in an important region that is part of the site where the enzyme binds the inhibitor. The swap of a tripeptide in this region was not sufficient to change the specificity of the two inhibitors towards their respective enzymes. The three-dimensional structure of the alph...Continue Reading

Citations

Nov 3, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Isabelle ZauggFrancesca Sparvoli
Nov 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Célia R Carlini, Maria Fátima Grossi-de-Sá
Mar 25, 2000·Insect Biochemistry and Molecular Biology·A ValenciaM J Chrispeels
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·J IulekM F Grossi de Sá
Feb 22, 2002·European Journal of Biochemistry·Octávio L FrancoMaria F Grossi-De-Sá
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·R L MortonT J Higgins
Aug 11, 2001·Molecular Plant-microbe Interactions : MPMI·A M Fakhoury, C P Woloshuk
Oct 11, 2005·FEBS Letters·Charles S A DaylerMaria F Grossi-de-Sá
Sep 4, 2007·Plant Physiology and Biochemistry : PPB·A M Guzman-PartidaL Vazquez-Moreno
Aug 27, 2005·Journal of Enzyme Inhibition and Medicinal Chemistry·Ludmila ZajoncovaPavel Pec
Jul 29, 2004·Journal of Economic Entomology·Curtis B HillGlen L Hartman
Nov 8, 2015·The Journal of Biological Chemistry·Phuong Quoc Thuc NguyenJames P Tam
Nov 30, 2012·Biopolymers·Arlete Beatriz Becker-Ritt, Célia Regina Carlini
Oct 12, 2018·International Journal of Insect Science·Jean-Luc Da Lage
Jan 7, 2018·International Journal of Biological Macromolecules·Abinaya BalasubramanianPachaiappan Raman
Mar 7, 2008·Journal of Agricultural and Food Chemistry·Arnubio Valencia-JiménezMaria Fátima Grossi-De-Sá

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