Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin.

Journal of Bacteriology
Melinda J MayerMichael J Gasson

Abstract

Clostridium difficile infection is increasing in both frequency and severity, with the emergence of new highly virulent strains highlighting the need for more rapid and effective methods of control. Here, we show that bacteriophage endolysin can be used to inhibit and kill C. difficile. The genome sequence of a novel bacteriophage that is active against C. difficile was determined, and the bacteriophage endolysin gene was subcloned and expressed in Escherichia coli. The partially purified endolysin was active against 30 diverse strains of C. difficile, and importantly, this group included strains of the major epidemic ribotype 027 (B1/NAP1). In contrast, a range of commensal species that inhabit the gastrointestinal tract, including several representatives of the clostridium-like Firmicutes, were insensitive to the endolysin. This endolysin provides a platform for the generation of both therapeutic and detection systems to combat the C. difficile problem. To investigate a method for the protected delivery and production of the lysin in the gastrointestinal tract, we demonstrated the expression of active CD27L endolysin in the lactic acid bacterium Lactococcus lactis MG1363.

References

Feb 1, 1985·Journal of Clinical Microbiology·D E MahonyK B Easterbrook
Jun 1, 1983·Journal of Clinical Microbiology·T L SellF R Fekety
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·D NelsonV A Fischetti
Aug 23, 2002·Nature·Raymond SchuchVincent A Fischetti
Nov 14, 2003·The Journal of Antimicrobial Chemotherapy·Isabel JadoUNKNOWN Spanish Pneumococcal Infection Study Network
Feb 5, 2005·Applied and Environmental Microbiology·Shan GohBarbara J Chang
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Apr 16, 2005·Clinical Microbiology Reviews·Daniel E Voth, Jimmy D Ballard
Jun 25, 2005·Bioinformatics·Tim J CarverJulian Parkhill
Jun 28, 2005·Current Opinion in Microbiology·Martin J Loessner
Jul 16, 2005·Current Opinion in Microbiology·Sherwood R Casjens
Aug 30, 2005·Trends in Microbiology·Vincent A Fischetti
Apr 7, 2006·Applied and Environmental Microbiology·David M DonovanDavid G Pritchard
May 24, 2006·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Henri BraatLothar Steidler
Nov 7, 2006·Applied and Environmental Microbiology·Peter Sass, Gabriele Bierbaum
Nov 23, 2006·Trends in Biotechnology·Nicola K PettyGeorge P C Salmond
Nov 23, 2006·Annals of Internal Medicine·John G Bartlett
Dec 23, 2006·Expert Opinion on Drug Delivery·Saravanan YuvarajNicolaas A Bos
Dec 28, 2006·The Journal of Infectious Diseases·Sean B HanniffyJerry M Wells
Jul 21, 2007·Internal Medicine Journal·B ElliottT V Riley
Sep 25, 2007·Applied and Environmental Microbiology·Louis-Charles Fortier, Sylvain Moineau
Oct 2, 2007·Current Opinion in Microbiology·Juan A HermosoPedro García
Oct 24, 2007·The Journal of Infectious Diseases·Mohammad RashelShigenobu Matsuzaki

❮ Previous
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Citations

Jun 10, 2009·British Medical Bulletin·O Martin Williams, Robert C Spencer
Feb 22, 2012·Applied and Environmental Microbiology·Lorena Rodríguez-RubioPilar García
Jun 19, 2012·Applied and Environmental Microbiology·Jinyu ShanMartha R J Clokie
Aug 6, 2013·Applied and Environmental Microbiology·K R HargreavesM R J Clokie
Aug 28, 2012·Applied and Environmental Microbiology·Mathieu Meessen-PinardLouis-Charles Fortier
Mar 29, 2011·Journal of Bacteriology·Ognjen SekulovicLouis-Charles Fortier
Oct 28, 2009·BMC Microbiology·Rob LavigneAndrew M Kropinski
May 25, 2012·PloS One·Janet Y NaleMartha R J Clokie
Jan 30, 2014·PloS One·Katherine R HargreavesMartha R J Clokie
Jan 12, 2013·Current Protein & Peptide Science·Zuzanna Drulis-KawaRob Lavigne
Aug 15, 2013·Applied Microbiology and Biotechnology·Teresa GervasiMelinda J Mayer
Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Feb 23, 2013·Expert Opinion on Drug Discovery·Mario ZuccaDianella Savoia
Jul 31, 2013·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Janet T LeeSara J Shumway
Jun 15, 2011·Anaerobe·Govind RevathiRial D Rolfe
May 24, 2013·Journal of Medical Microbiology·Rachel WilliamsPeter Mullany
Feb 18, 2011·Bioengineered Bugs·Mark FentonAidan Coffey
Oct 16, 2012·Virology·Marine Henry, Laurent Debarbieux
May 9, 2014·Frontiers in Microbiology·Katherine R Hargreaves, Martha R J Clokie
Aug 24, 2010·FEMS Microbiology Letters·Marine HenryJim M O'Mahony
Oct 28, 2016·Biotechnology and Bioengineering·Krunal K MehtaRavi S Kane
Sep 24, 2015·Antimicrobial Agents and Chemotherapy·Qiong WangVincent A Fischetti
Dec 9, 2015·Antimicrobial Agents and Chemotherapy·Janet Y NaleMartha R J Clokie
Dec 17, 2014·Applied and Environmental Microbiology·Ognjen Sekulovic, Louis-Charles Fortier
Feb 18, 2014·Applied and Environmental Microbiology·Ognjen SekulovicLouis-Charles Fortier
Jan 18, 2015·Applied and Environmental Microbiology·Minsuk Kong, Sangryeol Ryu
Jul 26, 2017·Science Progress·Jude AjueborAidan Coffey
Jul 20, 2019·Expert Review of Anti-infective Therapy·Ewa Jończyk-MatysiakAndrzej Górski

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