Molecular characterization of a conformational epitope of hen egg white lysozyme by differential chemical modification of immune complexes and mass spectrometric peptide mapping

Bioconjugate Chemistry
W FiedlerM Przybylski

Abstract

A new approach for the characterization of conformationally dependent epitope structures in protein antigens is described using differential chemical modification of immune complexes in combination with mass spectrometric peptide mapping analysis. Well-established methods for epitope characterization are frequently not applicable to conformationally dependent epitopes, and direct methods of structure analysis such as X-ray crystallography of immune complexes have been successful only in a few cases. Our approach combines tertiary structure-selective chemical modification of immune complexes with the molecular characterization of reaction products by mass spectrometric peptide mapping. The comparison of the modification pattern of free and antibody-bound antigen provides the identification of residues protected from modification by the antibody. These residues hence are characterized as part of the epitope structure. The well-characterized hen egg white lysozyme and a corresponding monoclonal IgM-type antibody were investigated as a model system. Specific modification reactions for arginine, lysine, and tyrosine residues were performed, and the modification sites in free and antibody-bound antigen were determined by mass spectro...Continue Reading

References

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Citations

Jun 4, 1999·Progress in Biophysics and Molecular Biology·K A Resing, N G Ahn
Feb 2, 1999·Journal of the American Society for Mass Spectrometry·T A FliggeM Przybylski
Jun 21, 2007·Chemical Communications : Chem Comm·Nicola McMillanLeroy Cronin
Dec 31, 2015·Expert Review of Proteomics·Danilo DonnarummaNathalie Norais
Nov 19, 2008·Mass Spectrometry Reviews·Vanessa Leah Mendoza, Richard W Vachet
Sep 29, 2004·Biochemical and Biophysical Research Communications·Jirí SantrůcekMilan Kodícek
Sep 29, 2004·Biochemical and Biophysical Research Communications·Martin StrohalmMilan Kodícek
Jul 4, 2012·Journal of Molecular Biology·W E RunningJ P Reilly
Aug 25, 2011·Journal of the American Chemical Society·Adrian MoiseHans-Joachim Gabius
Oct 8, 2005·Expert Review of Proteomics·Christine Hager-Braun, Kenneth B Tomer
Jun 15, 2007·Analytical Biochemistry·Xiaohui LiuJames P Reilly
Sep 5, 2003·Analytical and Bioanalytical Chemistry·Marcus MachtMichael Przybylski
Jul 13, 2016·Mass Spectrometry Reviews·Kwabena F M OpuniMichael O Glocker
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E O HochleitnerK B Tomer
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Jan 31, 2009·Journal of Proteome Research·William E Running, James P Reilly
Nov 4, 2006·Journal of Proteome Research·Richard L BeardsleyJames P Reilly
Jan 25, 2002·Analytical Chemistry·Michele A KellyPhilip J Rosner
Dec 1, 2021·Journal of the American Chemical Society·Lukáš FojtíkPetr Novák
Dec 22, 2021·Analytical Chemistry·Catherine Y TremblayRichard W Vachet
Dec 31, 2021·Chemical Reviews·Evgeniy V Petrotchenko, Christoph H Borchers

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