Molecular characterization of a glyoxysomal long chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin.

The Journal of Biological Chemistry
H HayashiM Nishimura

Abstract

A cDNA clone for pumpkin acyl-CoA oxidase (EC 1.3.3.6; ACOX) was isolated from a lambdagt11 cDNA library constructed from poly(A)+ RNA extracted from etiolated cotyledons. The inserted cDNA clone contains 2313 nucleotides and encodes a polypeptide of 690 amino acids. Analysis of the amino-terminal sequence of the protein indicates that the pumpkin acyl-CoA oxidase protein is synthesized as a larger precursor containing a cleavable amino-terminal presequence of 45 amino acids. This presequence shows high similarity to the typical peroxisomal targeting signal (PTS2). Western blot analysis following cell fractionation in a sucrose gradient revealed that ACOX is localized in glyoxysomes. A partial purification of ACOX from etiolated pumpkin cotyledons indicated that the ACOX cDNA codes for a long chain acyl-CoA oxidase. The amount of ACOX increased and reached to the maximum activity by day 5 of germination but decreased about 4-fold on the following days during the subsequent microbody transition from glyoxysomes to leaf peroxisomes. By contrast, the amount of mRNA was already high at day 1 of germination, increased by about 30% at day 3, and faded completely by day 7. These data indicated that the expression pattern of ACOX was v...Continue Reading

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Citations

Nov 4, 2011·Plant Molecular Biology·Bibi Rafeiza KhanBethany K Zolman
Jan 5, 2002·Progress in Lipid Research·Ian A Graham, Peter J Eastmond
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M A HooksI A Graham
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Jul 4, 2006·The Plant Journal : for Cell and Molecular Biology·Shoji ManoMikio Nishimura
Aug 18, 2004·Journal of Molecular Biology·Oleh I PetrivRichard A Rachubinski
Sep 29, 2006·Biochimica Et Biophysica Acta·Makoto Hayashi, Mikio Nishimura
Feb 24, 2006·Vitamins and Hormones·Shoji Mano, Mikio Nishimura
Apr 23, 1999·The Journal of Biological Chemistry·H HayashiM Nishimura
Nov 22, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Markus Kunze
Jul 13, 2000·Journal of Molecular Biology·D M ChudzikW G Hol
Aug 18, 2004·Plant Physiology·Anthony L ContentoDiane C Bassham

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