Molecular characterization of a major serotype M49 group A streptococcal DNase gene (sdaD).

Infection and Immunity
A PodbielskiJ Weber-Heynemann

Abstract

Group A streptococci (GAS) express up to four types of secreted DNases. Although GAS infections are correlated with the production of anti-DNase B antibodies, the roles of DNases in the pathogenesis of GAS infections remain unclear. From a lambda library of serotype M49 strain CS101 GAS genome, a 2,147-bp fragment expressing DNase activity on an indicator agar was identified and sequenced. One 1,155-bp open reading frame (ORF) was identified in this fragment. This ORF was found to be 48% identical on the amino acid level to group C streptococcal DNase (Sdc). The regions of highest homology corresponded to amino acid residues that were also identified as part of the active site in staphylococcal nuclease. Transcription analysis revealed a specific 1.3-kb mRNA, which corresponded to the size predicted by the promoter and transcription termination signature sequences and indicated a monocistronic mode of transcription. Allelic replacement of the ORF rendered a M49 mutant devoid of extracellular DNase activity when cultured on indicator agar. Virulence parameters such as resistance to phagocytosis were not affected by the mutation. The sda gene was cloned and expressed in Escherichia coli as a thioredoxin fusion. By performing Ouch...Continue Reading

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Citations

Mar 26, 2003·Infection and Immunity·Kimmo VirtanevaJames M Musser
Jan 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul SumbyJames M Musser
Apr 23, 2005·Infection and Immunity·Chih-Hung WangJiunn-Jong Wu
Jun 6, 2009·FEMS Microbiology Letters·Alexander N SuvorovJoseph J Ferretti
Feb 13, 2001·Infection and Immunity·M S ChausseeJ M Musser
Feb 26, 2000·Infection and Immunity·G L DarmstadtC E Rubens
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J FerrettiR McLaughlin
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jovanka M VoyichFrank R DeLeo
Jun 26, 2012·Journal of Bacteriology·Lauren Mashburn-WarrenMichael J Federle
Apr 6, 2002·FEMS Immunology and Medical Microbiology·Lara SolerJosep Guarro
Jan 5, 1999·Clinical and Diagnostic Laboratory Immunology·A ErikssonM Norgren
Mar 16, 2019·Microbiology Spectrum·Horst Malke
Aug 7, 2021·Frontiers in Cardiovascular Medicine·M Taariq SalieMark E Engel

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