Molecular characterization of a nonsecreting myeloma mutant

European Journal of Immunology
Y ArgonC Milstein

Abstract

The nonsecreting myeloma mutant, NSII/1, derived from MOPC 21 a mouse myeloma cell line, does not produce L chains and expresses a smaller than normal gamma 1 heavy chain, H* (Cowan, Secher and Milstein, J. Mol. Biol. 1974. 90: 691). The free H* is not secreted, but secretion can be rescued in hybrids expressing a light chain. By sequencing L and H chain-cloned cDNA derived from NSII/1 we find only one light chain mRNA expressed which belongs to an aberrantly rearranged kappa gene. We also find that the H* heavy chain is the result of a point mutation. H* mRNA contains an A instead of the G found in wild type at position 1125. The corresponding tryptophan 406 becomes a chain termination, giving rise to a 67 amino acid deletion at the C-terminus. The H* is core-glycosylated, but its state of assembly is abnormal: H* forms high molecular weight, S-S-bonded complexes. The results are discussed with reference to the putative toxic properties of free heavy chains and the failure of some chains to be secreted.

References

Jul 1, 1978·The Journal of Experimental Medicine·G E SonensheinM L Gefter
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1979·Immunological Reviews·D BaltimoreO N Witte
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·C MilsteinC D Wilde
Jan 1, 1977·Immunological Reviews·D S SecherK Adetugbo
Sep 1, 1977·Journal of Molecular Biology·K Adetugbo, C Milstein
May 15, 1978·Journal of Molecular Biology·K Adetugbo, C Milstein
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Feb 1, 1972·The Biochemical Journal·J Svasti, C Milstein
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Köhler

❮ Previous
Next ❯

Citations

Jul 1, 1986·Molecular Immunology·A BakhshiS J Korsmeyer
Sep 29, 1999·Immunology Today·F J Stevens, Y Argon
Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Colleen M DoyleEline T Luning Prak
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I G Haas, M R Wabl
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J L Dul, Y Argon
Oct 6, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine Zhu, Ellen Hsu
Nov 1, 1987·European Journal of Immunology·A AlanenR M Franklin
Mar 1, 1985·European Journal of Immunology·A AlanenR M Franklin
Jan 1, 1984·Medical Oncology and Tumor Pharmacotherapy·G KöhlerD Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.