Molecular characterization of a novel amastigote stage specific Class I nuclease from Leishmania major

International Journal for Parasitology
S FarajniaF Mahboudi

Abstract

Leishmania parasites like other kinetoplastids are unable to synthesize purines de novo and so are reliant on a salvage pathway for recycling ribonucleotides. A stage specific class one nuclease enzyme, 3'-Nucleotidase/nuclease, has been implicated in salvage of preformed purines in Leishmania insect stage promastigote via hydrolysis of 3'-nucleotides and nucleic acids. Although a similar activity is known to exist in amastigotes which reside in infected mammalian cells, the homologous gene and the corresponding protein responsible for carrying out this function have not been well characterized. Using primers specific for conserved regions of trypanosomatid class one nucleases, a gene encoding a novel class one nuclease from amastigotes of Leishmania major (LmaC1N) was cloned and sequenced. The coding sequence consists of 951 bp encoding a 316 amino acid protein with a predicted molecular mass of 35,300 Da. Analysis of the deduced amino acid sequence showed that LmaC1N is highly homologous to other class I nucleases and contains all five conserved regions reported for promastigotes 3'-Nucleotidase/nuclease. Analysis by reverse transcriptase polymerase chain reaction and Western blotting demonstrated that expression of LmaC1N ge...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1992·The American Journal of Tropical Medicine and Hygiene·M GroglE D Franke
Jul 1, 1992·The Biochemical Journal·G O Gbenle, D M Dwyer
Jul 11, 1991·Molecular and Biochemical Parasitology·T A CampbellM Gottlieb
Sep 15, 1991·European Journal of Biochemistry·K MaekawaF Sakiyama
Jul 1, 1991·Journal of Biochemistry·A IwamatsuF Sakiyama
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B L Vallee, D S Auld
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M GottliebD M Dwyer
Apr 1, 1987·Molecular and Biochemical Parasitology·H F Hassan, G H Coombs
Feb 1, 1984·Molecular and Biochemical Parasitology·D M Dwyer, M Gottlieb
Nov 1, 1984·Molecular and Biochemical Parasitology·D J Hammond, W E Gutteridge
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Sep 29, 2000·BMJ : British Medical Journal·L J RobertsS J Foote
Feb 19, 2002·International Journal for Parasitology·William F SopwithPaul A Bates

❮ Previous
Next ❯

Citations

Mar 14, 2014·Molecular Microbiology·Aurélien DupéBarbara Papadopoulou
Jul 15, 2011·Enzyme Research·Safar FarajniaSoheila Montazer Saheb
Aug 11, 2011·Experimental Parasitology·Rafael Paletta-SilvaJosé Roberto Meyer-Fernandes
Feb 6, 2007·The Journal of Biological Chemistry·Manju B Joshi, Dennis M Dwyer
May 5, 2020·Nucleic Acids Research·Tiago R FerreiraPegine B Walrad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.