PMID: 9436997Mar 14, 1998Paper

Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome components

Molecular Biology of the Cell
Marion S Schmidt-ZachmannA Krämer

Abstract

We report the identification and molecular characterization of a novel type of constitutive nuclear protein that is present in diverse vertebrate species, from Xenopus laevis to human. The cDNA-deduced amino acid sequence of the Xenopus protein defines a polypeptide of a calculated mass of 146.2 kDa and a isoelectric point of 6.8, with a conspicuous domain enriched in the dipeptide TP (threonine-proline) near its amino terminus. Immunolocalization studies in cultured cells and tissues sections of different origin revealed an exclusive nuclear localization of the protein. The protein is diffusely distributed in the nucleoplasm but concentrated in nuclear speckles, which represent a subnuclear compartment enriched in small nuclear ribonucleoprotein particles and other splicing factors, as confirmed by colocalization with certain splicing factors and Sm proteins. During mitosis, when transcription and splicing are downregulated, the protein is released from the nuclear speckles and transiently dispersed throughout the cytoplasm. Biochemical experiments have shown that the protein is recovered in a approximately 12S complex, and gel filtration studies confirm that the protein is part of a large particle. Immunoprecipitation and Wes...Continue Reading

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Citations

Apr 6, 2000·Molecular Biology of the Cell·R F ZirwesM S Schmidt-Zachmann
Mar 13, 2003·Molecular Biology of the Cell·Kannanganattu V PrasanthDavid L Spector
Jan 27, 2004·Molecular Biology of the Cell·Jens EilbrachtMarion S Schmidt-Zachmann
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J Eilbracht, M S Schmidt-Zachmann
Feb 16, 2010·Biochemical and Biophysical Research Communications·Ko EtoShin-ichi Abe
Apr 12, 2005·European Journal of Cell Biology·Jens EilbrachtMarion S Schmidt-Zachmann
Dec 17, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Paul GutweinGlen Kristiansen
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Feb 1, 2007·Journal of Cell Science·Yannis Robin-LespinasseMuriel Le Romancer
Aug 19, 2004·Journal of Cell Science·Dobrila NesicAngela Krämer

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