Molecular Characterization of a Novel Endornavirus Conferring Hypovirulence in Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA Strain GD-2

Viruses
Li ZhengErxun Zhou

Abstract

The complete sequence and genome organization of a novel Endornavirus from the hypovirulent strain GD-2 of Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight, were identified using a deep sequencing approach and it was tentatively named as Rhizoctonia solani endornavirus 1 (RsEV1). It was composed of only one segment that was 19,936 bp in length and was found to be the longest endornavirus genome that has been reported so far. The RsEV1 genome contained two open reading frames (ORFs): ORF1 and ORF2. ORF1 contained a glycosyltransferase 1 domain and a conserved RNA-dependent RNA polymerase domain, whereas ORF2 encoded a conserved hypothetical protein. Phylogenetic analysis revealed that RsEV1 was phylogenetically a new endogenous RNA virus. A horizontal transmission experiment indicated that RsEV1 could be transmitted from the host fungal strain GD-2 to a virulent strain GD-118P and resulted in hypovirulence in the derivative isogenic strain GD-118P-V1. Metabolomic analysis showed that 32 metabolites were differentially expressed between GD-118P and its isogenic hypovirulent strain GD-118P-V1. The differential metabolites were mainly classified as organic acids, amino acids, carbohydrates, and the intermediate pr...Continue Reading

References

Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·E V Koonin, V V Dolja
Jan 25, 2000·The Journal of General Virology·E E StraussS M Tavantzis
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Alisdair R FernieLothar Willmitzer
Sep 28, 2004·The Journal of General Virology·Nicolas Markine-GoriaynoffAlain Vanderplasschen
Dec 23, 2004·Bioinformatics·Joachim KopkaDirk Steinhauser
Aug 3, 2005·Nature Reviews. Microbiology·Donald L Nuss
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L K Grill, S J Garger
Oct 24, 2008·Phytopathology·M D WuH-C Huang
Oct 25, 2008·Nature·Jeremy K Nicholson, John C Lindon
Jan 24, 2009·Molecular Plant Pathology·Michael N PearsonKieren Arthur
Apr 22, 2009·Annual Review of Microbiology·Jiqiang LingMichael Ibba
Apr 30, 2009·Annual Review of Phytopathology·Said A Ghabrial, Nobuhiro Suzuki
Nov 12, 2009·FEBS Letters·Rajat BanerjeeMichael Ibba
Jul 22, 2011·The Journal of General Virology·Marilyn J RoossinckRodrigo A Valverde
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xiao YuDaohong Jiang
Jul 9, 2014·Annual Review of Phytopathology·Jiatao Xie, Daohong Jiang
Mar 17, 2015·Virology·Said A GhabrialNobuhiro Suzuki
May 16, 2018·Nucleic Acids Research·Jasmine ChongJianguo Xia

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Citations

Dec 6, 2019·Viruses·Assane Hamidou AbdoulayeIoly Kotta-Loizou
Mar 7, 2021·Viruses·Armelle MaraisMarie-France Corio-Costet
Apr 27, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ergin SahinEmre Keskin

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Methods Mentioned

BETA
electrophoresis
cDNA library construction
PCR
Illumina sequencing
PCA

Software Mentioned

XCMS
Molecular Evolutionary Genetics Analysis ( MEGA )
SAS
Clustal
SIMCA
BLASTp
MetaboAnalyst

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