Molecular characterization of a plasmid-borne (pTC82) chloramphenicol resistance determinant (cat-TC) from Lactobacillus reuteri G4

Plasmid
C F LinT C Chung

Abstract

Lactobacillus reuteri G4 contains a 7.0-kb plasmid (pTC82) encoding resistance to chloramphenicol (Cm). Determination of the nucleotide sequence of the genetic determinant (cat-TC) encoding resistance to Cm on pTC82 revealed an open reading frame for a 238-amino-acid Cm acetyltransferase (CAT) monomer. This structural cat gene, 714 bp in length, was highly related (ca. 95% nucleotide and ca. 81% amino acid identity) to the 648-bp cat gene from Staphylococcus aureus plasmid pC194. A total of 6 bp transversions and 4 bp deletions was observed along the whole DNA sequence of cat-TC compared to that of cat-pC194. To determine the activity of the putative cat-TC gene, recombinant plasmid pUC8217 containing the cat determinant from pTC82 was subjected to a maxicell analysis. The observed molecular mass of the synthesized protein, based on electrophoretic mobility, was in reasonable agreement with the 27.3 kDa predicted from the DNA sequence. This is the first reported nucleotide sequence of a Cm-resistance determinant from L. reuteri and also the first evidence of adding Lactobacillus to the list of versatile bacterial genera which naturally acquire the cat-pC194 gene in the microbial ecological system.

Citations

Jan 10, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David R Snydman
Apr 9, 2008·Applied and Environmental Microbiology·Fang FangPaul W O'Toole
Jan 30, 2007·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·M Del PianoL Capurso
Apr 10, 2007·Food Microbiology·Mohammed Salim AmmorBaltasar Mayo
Jun 16, 2011·Mycoses·Daniela Mailänder-SánchezMartin Schaller
Sep 29, 2006·International Journal of Food Microbiology·Cristiana GarofaloFrancesca Biavasco
Nov 18, 2005·International Journal of Food Microbiology·Shalini Mathur, Rameshwar Singh
May 24, 2015·International Journal of Food Microbiology·Maria João Fraqueza
Nov 13, 2004·FEMS Microbiology Reviews·Stefan SchwarzAxel Cloeckaert
Apr 30, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shira Doron, David R Snydman
Dec 13, 2017·Frontiers in Immunology·Aaron LernerRustam Aminov
Jun 1, 2010·Beneficial Microbes·V Karthikeyan, S W Santosh
Apr 14, 2019·Microorganisms·Aaron LernerTorsten Matthias
Jul 26, 2017·Probiotics and Antimicrobial Proteins·Mohamed ZommitiMounir Ferchichi
Mar 28, 2019·Frontiers in Microbiology·José M Castro-GonzálezDiana Castro-Sandoval
Mar 8, 2007·Journal of Food Protection·Maria EgervärnSven Lindgren
Jun 8, 2021·The British Journal of Nutrition·Mona HegazyAhmed Abdelghani

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