Molecular characterization of a ryanodine receptor gene in the rice leaffolder, Cnaphalocrocis medinalis (Guenée).

PloS One
Jianjun WangXianchun Li

Abstract

Ryanodine receptors (RyRs) are the targets of two novel classes of synthetic insecticidal chemicals, phthalic acid diamides and anthranilic diamides. Isolation of full-length RyR cDNAs is a critical step towards the structural and functional characterization of insect RyRs and an understanding of the molecular mechanisms underlying the species selective toxicity of diamide insecticides. However, there has been little research on the insect RyR genes due to the high molecular weight of the RyR proteins. In this study, we isolated a full-length RyR cDNA (named as CmRyR) from Cnaphalocrocis medinalis, an important rice pest throughout Southeast Asia. The composite CmRyR gene contains an ORF of 15264 bp encoding a protein of 5087 amino acid residues, which shares 79% overall identity with its Drosophila melanogaster homologue. All hallmarks of the RyR proteins are conserved in the CmRyR protein, suggesting that CmRyR is a structural and functional analogue of known RyRs. A multiple sequence alignment illustrates that the insect RyRs share high levels of amino acid sequence identity at the the COOH-terminal region. However, the amino acid residues analogous to the CmRyR residues N(4922), N(4924), N(4935), L(4950), L(4981), N(5013) a...Continue Reading

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Citations

Dec 5, 2013·Genome Génome / Conseil National De Recherches Canada·Yonglei LiuKejian Lin
Apr 23, 2013·Developmental and Comparative Immunology·Shunfan WuGongyin Ye
Nov 26, 2013·Pesticide Biochemistry and Physiology·Jian WangJianjun Wang
Apr 7, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wen-Tao YangChun-Feng Wang
Oct 21, 2016·Journal of Economic Entomology·Rebecca A Schmidt-Jeffris, Brian A Nault
Aug 16, 2016·Journal of Economic Entomology·R A WhalenD D Reisig

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Datasets Mentioned

BETA
JQ799046
DJ085056
AET09964
EFN78897
BAA41471
EAT44864
BAA08309
CAA33279
AAD01425
EHJ77857

Methods Mentioned

BETA
PCR

Software Mentioned

ContigExpress
CLUSTALW
Vector NTI Advance
MEGA5

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