Molecular characterization of a stable antisense chalcone synthase phenotype in strawberry (Fragaria x ananassa)

Journal of Agricultural and Food Chemistry
Stefan LunkenbeinElma M J Salentijn

Abstract

An octaploid (Fragaria x ananassa cv. Calypso) genotype of strawberry was transformed with an antisense chalcone synthase (CHS) gene construct using a ripening related CHS cDNA from Fragaria x ananassa cv. Elsanta under the control of the constitutive CaMV 35S promoter via Agrobacterium tumefaciens. Out of 25 transgenic lines, nine lines showed a reduction in CHS mRNA accumulation of more than 50% as compared to the untransformed cv. Calypso control. The antisense CHS construct was found to be integrated into the genome, with a copy number ranging from one to four. The pigmentation of the fruit was only affected when less than 5% of the control CHS expression level was detected. A stable antisense phenotype over a period of 4 years was obtained in the primary transgenic lines at a rate of 1:20. As a consequence of the reduced activity of CHS, the levels of anthocyanins, flavonols, and proanthocyanidins were downregulated and precursors of the flavonoid pathway were shunted to the phenylpropanoid pathway leading to highly increased levels of cinnamoyl glucose (520% of control), caffeoyl glucose (816% of control), and feruloyl glucose (1092% of control) as well as p-coumaryl alcohol (363% of control) and p-coumaryl-1-acetate (107...Continue Reading

References

Jul 1, 1995·Transgenic Research·F A van EngelenW J Stiekema
Jul 30, 1999·Nature Structural Biology·J Schröder
Jul 4, 2001·European Journal of Biochemistry·H MoritaI Abe
Feb 14, 2002·Plant Physiology·Silvia Jiménez-BermúdezJosé A Mercado
Sep 27, 2002·Journal of Experimental Botany·Asaph Aharoni, Ann P O'Connell
Sep 27, 2002·Journal of Experimental Botany·M E VerhoeyenS Colliver
Mar 7, 2003·Phytochemistry·Amrita Kumar, Brian E Ellis
Apr 26, 2003·Annals of Botany·Jeffrey S Amthor
Apr 1, 1996·Plant Cell Reports·I El MansouriM A Quesada

❮ Previous
Next ❯

Citations

Mar 3, 2012·Transgenic Research·Giorgio Gambino, Ivana Gribaudo
Jun 22, 2011·Metabolic Engineering·Thomas HoffmannWilfried Schwab
Feb 19, 2008·Biotechnology Advances·Yonghua QinShanglong Zhang
Sep 11, 2013·Biotechnology Journal·Irene Aragüez, Victoriano Valpuesta
Feb 13, 2013·The Plant Journal : for Cell and Molecular Biology·Andrew P DareRoger P Hellens
Oct 28, 2014·Frontiers in Plant Science·Su-Ying YehWilfried Schwab
Nov 4, 2016·Journal of Agricultural and Food Chemistry·Priyanka R ChaudharyBhimanagouda S Patil
Mar 19, 2014·Journal of Agricultural and Food Chemistry·Lucia LandiGianfranco Romanazzi
Apr 4, 2017·The Plant Journal : for Cell and Molecular Biology·Andrew P DareRoss G Atkinson
Jun 5, 2013·Plant Signaling & Behavior·Andrew P Dare, Roger P Hellens
Feb 10, 2016·The Plant Journal : for Cell and Molecular Biology·Chuankui SongWilfried Schwab
Mar 1, 2020·Science China. Life Sciences·Feng ZhangJie Luo
Jan 12, 2011·Journal of Agricultural and Food Chemistry·Klaus OlbrichtChristine Grafe
Nov 25, 2017·Journal of Agricultural and Food Chemistry·Mwafaq IbdahDavid R Gang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.