PMID: 7543861Aug 1, 1995Paper

Molecular characterization of a virulence-associated epitope on the lipopolysaccharide of Legionella pneumophila serogroup 1

Epidemiology and Infection
J H HelbigU Zähringer

Abstract

For identification of lipopolysaccharide (LPS)-associated epitopes of Legionella pneumophila serogroup 1, LPS of strain Philadelphia 1 was investigated using monoclonal antibodies (MAbs). The O-specific chain of LPS is a homopolymer of 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-D-glycero- L-galacto- nonulosonic acid. At least four immunoaccessible epitopes were recognized by different MAbs on the intact LPS. After O-deacetylation of LPS, the reactivity of one of the MAbs (MAb 3/1) was lost, indicating thus that the corresponding epitope is associated with the 8-O-acetyl group. Since the reactivity pattern of the MAb 3/1 is identical with those of the MAb 2 which was considered as a virulence marker for serogroup 1, this epitope may be involved in mediating virulence in L. pneumophila. Four MAbs specific to strains of serogroup 1 other than the monoclonal subtype Philadelphia recognized epitopes on the O-deacetylated LPS of strain Philadelphia 1 and, therefore, the virulence-associated epitope blocks recognition of the immunodeterminants that are accessible on the intact LPS of the strains lacking this epitope.

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Citations

Jul 7, 1998·The Journal of Experimental Medicine·E LünebergM Frosch
Apr 3, 2012·Applied and Environmental Microbiology·J A C SchalkA M de Roda Husman
May 23, 2006·Infection and Immunity·Esteban Fernandez-MoreiraMichele S Swanson
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Jan 11, 2020·Frontiers in Microbiology·Marta Palusinska-SzyszBozena Kowalczyk
Feb 6, 2021·International Journal of Molecular Sciences·Bozena KowalczykMarta Palusinska-Szysz

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