Molecular characterization of adult-colonizing Streptococcus agalactiae from an area-based surveillance study in Romania.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
C-R UseinM Strãuţ

Abstract

One hundred and forty-eight colonizing isolates from adult Romanian women were conventionally serotyped and screened for antibiotic resistance. Capsular type assignment by multiplex polymerase chain reaction (PCR) was performed for nonserotypeable isolates. Tetracycline and macrolide resistance genes (tetM, tetO, tetL, ermA, ermB, and mefA) including tetM gene association with conjugative elements of the Tn916 family were searched. Molecular typing included PCR screening for major surface protein antigen genes (bac, bca, alp1, alp2/3, alp4, and rib), mobile genetic elements (GBSi1 and IS1548), and rapid detection of hypervirulent clone ST-17. Genetic diversity was assessed by pulsed field gel electrophoresis (PFGE) analysis of SmaI macrorestriction patterns. Among the colonizing isolates studied, serotypes V and III predominated and high rates of tetracycline and macrolide resistance were observed. The tetM gene occurred in 140 tetracycline-resistant isolates and was associated with the int-Tn916 gene in 94 of them. Most of the isolates displayed a constitutive MLS(B) phenotype (38/46 isolates) and harbored the ermB gene. rib, alp2/3, and alp1 were the most common surface protein genes detected. Either IS1548 or GBSi1 intron we...Continue Reading

References

Nov 1, 1996·Antimicrobial Agents and Chemotherapy·J SutcliffeL Wondrack
Feb 15, 2000·Research in Microbiology·J MahillonM Chandler
Dec 21, 2000·Molecular Microbiology·F Martínez-Abarca, N Toro
Oct 2, 2002·Molecular Microbiology·Philippe GlaserFrank Kunst
Jan 25, 2003·Antimicrobial Agents and Chemotherapy·Claire PoyartPatrick Trieu-Cuot
Aug 29, 2003·Expert Opinion on Biological Therapy·Lawrence C Paoletti, Dennis L Kasper
Mar 9, 2004·Journal of Clinical Microbiology·Roberta CretiChristina von Hunolstein
Oct 2, 2004·Molecular Microbiology·Kelly S Doran, Victor Nizet
Nov 13, 2004·The Journal of Antimicrobial Chemotherapy·Brigitte MalbrunyRoland Leclercq
Jan 18, 2005·Clinical Microbiology Reviews·Gunnar LindahlThomas Areschoug
May 27, 2005·Antimicrobial Agents and Chemotherapy·Wehbeh WehbehJames J Rahal
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hervé TettelinClaire M Fraser
Jul 11, 2006·Microbes and Infection·Marie-Cécile LamyClaire Poyart
Mar 23, 2007·Journal of Clinical Microbiology·Claire PoyartPatrick Trieu-Cuot
May 29, 2007·Applied and Environmental Microbiology·Geneviève Héry-ArnaudLaurent Mereghetti
Nov 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A van BelkumUNKNOWN European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Epidemiological Markers (ESGEM)
Nov 24, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E PerssonB Trollfors
Sep 13, 2008·The Journal of Antimicrobial Chemotherapy·Anne-Sophie DomelierRoland Quentin
Dec 18, 2008·The Journal of Infectious Diseases·Immaculada MargaritGuido Grandi
Feb 19, 2009·Infectious Diseases in Obstetrics and Gynecology·Mei L CastorRuth Lynfield
Jun 2, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tami H SkoffStephanie J Schrag
Aug 14, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M GranlundUNKNOWN Swedish Working Group for the Prevention of Perinatal Group B Streptococcal Infections
Mar 23, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rosângela Stadnick Lauth de Almeida TorresLibera M Dalla-Costa
Jul 5, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M ImperiR Creti

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Citations

Mar 5, 2013·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Tatiana Castro Abreu PintoLeslie Claude Benchetrit
Nov 2, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N EskandarianS Amin Nordin
Jun 6, 2014·Epidemiology and Infection·P Sendi, S Fröhlicher
Mar 29, 2014·Memórias do Instituto Oswaldo Cruz·Codruţa-Romaniţa UseinMonica Străuţ

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