PMID: 1204613Nov 1, 1975Paper

Molecular characterization of an insect genome: Chironomus thummi

European Journal of Biochemistry
U Wobus

Abstract

DNA extracted from Chironomus thummi larvae was studied by isopycnic centrifugation in CsCl, thermal denaturation and DNA-DNA reassociation techniques. The mean G+C content of the C. thummi DNA is 28-29% as indicated both by centrifugation in CsCl and thermal denaturation. According to optical reassociation analysis of total DNA and of isolated DNA fractions the C. thummi genome is composed of at least four components. About 80% of the DNA is classified as unique with a kinetic complexity of nearly 7 X 10(10) daltons. 6-8% intermediate DNA exhibits a kinetic complexity slightly above 10(8) daltons with a mean repetition frequency of 35. 11-13% fast-reassociating DNA has a kinetic complexity slightly above 10(6) daltons with a mean repetition frequency of 6000. 3-5% of the DNA cannot be properly studied by the optical reassociation technique and probably contains inverted repeats. The thermal denaturation behaviour of isolated DNA fractions indicated that most of the repetitive sequences in the C. thummi genome are tightly interspersed.

References

Jan 1, 1973·International Review of Cytology·W Hennig
Nov 1, 1973·Biochemical Genetics·S H Turner, C D Laird
Jun 1, 1974·Analytical Biochemistry·G G Markov, I G Ivanov
Jul 10, 1974·Biochemical and Biophysical Research Communications·G Berencsi
May 28, 1972·Journal of Molecular Biology·R A Firtel, J Bonner
Nov 9, 1972·Biochimica Et Biophysica Acta·T R Hinds, R W Newburgh
Dec 1, 1973·The Quarterly Review of Biology·E H Davidson, R J Britten
Jan 1, 1973·Annual Review of Genetics·C D Laird
Feb 28, 1972·Journal of Molecular Biology·J R WuJ Bonner
Jun 15, 1973·Journal of Molecular Biology·E H DavidsonR J Britten
May 1, 1973·Cell Differentiation·J BalsamoF J Lara
Oct 1, 1972·Experimental Cell Research·R I Sachs, U Clever
Jul 1, 1971·Developmental Biology·E H DavidsonR J Britten
Feb 28, 1970·Journal of Molecular Biology·G BernardiP P Slonimski
Feb 1, 1965·Journal of Molecular Biology·Y MIYAZAWA, C A THOMAS
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER
Jun 1, 1962·Journal of Molecular Biology·C L SCHILDKRAUTP DOTY

❮ Previous
Next ❯

Citations

Jul 1, 1990·Molecular & General Genetics : MGG·U WobusN N Kolesnikov
Sep 9, 2011·Molecular Genetics and Genomics : MGG·Giampaolo ZampicininiGabriella Sella
Jul 10, 1982·Nucleic Acids Research·H BäumleinF C Kafatos
May 2, 1979·European Journal of Biochemistry·B Kaina, R Hinz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.