Molecular characterization of an endopolygalacturonase from Fusarium oxysporum expressed during early stages of infection

Applied and Environmental Microbiology
F I García-MacEiraM Isabel G Roncero

Abstract

The tomato vascular wilt pathogen Fusarium oxysporum f. sp. lycopersici produces an array of pectinolytic enzymes that may contribute to penetration and colonization of the host plant. Here we report the isolation of pg5, encoding a novel extracellular endopolygalacturonase (endoPG) that is highly conserved among different formae speciales of F. oxysporum. The putative mature pg5 product has a calculated molecular mass of 35 kDa and a pI of 8.3 and is more closely related to endoPGs from other fungal plant pathogens than to PG1, the major endoPG of F. oxysporum. Overexpression of pg5 in a bacterial heterologous system produced a 35-kDa protein with endoPG activity. Accumulation of pg5 transcript is induced by citrus pectin and D-galacturonic acid and repressed by glucose. As shown by reverse transcription-PCR, pg5 is expressed by F. oxysporum in tomato roots during the initial stages of infection. Targeted inactivation of pg5 has no detectable effect on virulence toward tomato plants.

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Citations

Jun 8, 2002·Fungal Genetics and Biology : FG & B·Holly L WilliamsWilliam E Hintz
May 31, 2013·Molecular Plant-microbe Interactions : MPMI·Gustavo Bravo-RuizM Isabel G Roncero
Dec 13, 2006·Molecular Plant-microbe Interactions : MPMI·Thomas K BaldwinKim E Hammond-Kosack
Mar 7, 2008·Molecular Plant-microbe Interactions : MPMI·Chih-Hang WuRuey-Fen Liou
Mar 9, 2010·Bioscience, Biotechnology, and Biochemistry·Shun OgawaKazuo Miyairi
Mar 17, 2010·Microbial Pathogenesis·Baozhen FengXiuguo Zhang
May 8, 2009·Genesis : the Journal of Genetics and Development·Wen Xiu SunXiu Guo Zhang
Nov 26, 2008·Journal of Basic Microbiology·Gisele Eleonora KikotTeresa Maria Alconada
Sep 1, 2003·Molecular Plant Pathology·Antonio Di PietroM Isabel G Roncero
Apr 30, 2009·Molecular Plant Pathology·Caroline B Michielse, Martijn Rep
Aug 19, 2008·Molecular Plant Pathology·Ana Lilia Martínez RochaM Isabel G Roncero
Nov 17, 2009·Molecular Microbiology·Wilfried Jonkers, Martijn Rep
Jan 1, 2006·Molecular Plant Pathology·Melvin D BoltonBerlin D Nelson
Oct 2, 2014·Molecular Plant-microbe Interactions : MPMI·Carmen Ruiz-RoldánM Isabel G Roncero
Oct 22, 2013·Fungal Genetics and Biology : FG & B·Heliana Argôlo Santos CarvalhoFabienne Micheli
Jan 30, 2019·Applied Microbiology and Biotechnology·Kevin SchmitzJ Philipp Benz
Jul 22, 2020·Phytopathology·He YangLi-Jun Ma
Apr 16, 2003·Molecular Microbiology·Zaira CaracuelAntonio Di Pietro
Jul 1, 2003·The New Phytologist·Ghislaine RecorbetClaude Alabouvette

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