Molecular characterization of ascorbate peroxidase (APX) and APX-related (APX-R) genes in Triticum aestivum L.

Genomics
Shivi TyagiSantosh Kumar Upadhyay

Abstract

Ascorbate peroxidases (APXs) are heme-dependent H2O2 scavenging enzymes involved in myriad biological processes. Herein, a total of 21 TaAPX and six TaAPX-R genes were identified from the A, B and D sub-genomes of Triticum aestivum L. The occurrence of three paralogous gene pairs with unequal evolutionary rate suggested functional divergence. The phylogenetic analysis formed four distinct clades having conserved gene and protein architecture, and sub-cellular localization. The tertiary structure analysis revealed the presence of helices and coils and residues involved in ligand binding. Transcriptional profiling of each TaAPX and TaAPX-R gene suggested their specific role during development and stress response. Modulated transcript expression and APX enzyme activity during various stress conditions indicated their role in stress response. Interaction analyses suggested their association with other genes, miRNAs and various legends. The present study reported numerous features of these genes, and may provide a platform for their detailed functional characterization in future studies.

References

Nov 25, 1988·Nucleic Acids Research·F Corpet
Jun 15, 1980·The Biochemical Journal·A BoverisA O Stoppani
Feb 3, 1998·Biochemical and Biophysical Research Communications·N WadaK Asada
Apr 5, 2000·The Journal of Biological Chemistry·R T Mullen, R N Trelease
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Kozi Asada
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Dec 16, 2004·Journal of Molecular Evolution·Felipe Karam TeixeiraMárcia Margis-Pinheiro
Jun 6, 2006·Proteins·Chin-Sheng YuJenn-Kang Hwang
Oct 17, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tsanko S GechevChristophe Laloi
Apr 25, 2007·Nucleic Acids Research·Warren A Kibbe
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
May 31, 2011·Nucleic Acids Research·Xinbin Dai, Patrick Xuechun Zhao
Jul 30, 2011·Journal of Structural Biology·Erik LundbergChristiane Funk
Jan 11, 2012·Plant Signaling & Behavior·Christophe DunandMarcia Margis-Pinheiro
Mar 7, 2012·Biochemical and Biophysical Research Communications·Zhang ZhangLin Dai
Oct 12, 2012·Molecular Biology Reports·Rehana KausarSetsuko Komatsu
Oct 24, 2012·Applied Biochemistry and Biotechnology·Khalid Rehman HakeemMuhammad Iqbal
Nov 28, 2012·Nucleic Acids Research·Nizar FawalChristophe Dunand
Dec 4, 2012·Nucleic Acids Research·Thomas NussbaumerManuel Spannagl
Feb 16, 2013·Genetics and Molecular Biology·Andréia CaverzanMárcia Margis-Pinheiro
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Jun 3, 2014·BMC Plant Biology·Fenglong SunYingyin Yao
Jul 19, 2014·Science·Frédéric ChouletCatherine Feuillet
Jul 19, 2014·Science·Thomas MarcussenOdd-Arne Olsen
Dec 17, 2014·Bioinformatics·Bo HuGe Gao

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