PMID: 9186939May 1, 1997Paper

Molecular characterization of avian reoviruses using nested PCR and nucleotide sequence analysis

Journal of Virological Methods
H J LiuB L Nielsen

Abstract

A nested polymerase chain reaction (PCR) with subsequent nucleotide sequence analysis identified and differentiated avian reoviruses (ARVs). PCR products amplified from the S1 gene segment of ARV of USA isolates were 738 and 342 bp, respectively. PCR products were conformed by Southern and dot blot hybridizations. The amplified cDNA fragments were cloned into the pUC18 vector and subjected to DNA sequencing. The nucleotide and deduced amino acid sequences of four USA (S1133, 1733, 2408, and CO8) and two Australian isolates (RAM-1 and SOM-4) were compared. Results of paired difference analysis and a predicted dendrogram revealed that USA isolates were closely related, but different from, Australian isolates. The deduced amino acid sequences of the N-terminal region of ARV sigma C showed a heptapeptide repeat of hydrophobic residues in all ARV isolates.

References

Jan 1, 1978·Preparative Biochemistry·J R Diaz-Ruiz, J M Kaper
Jun 1, 1990·Veterinary Microbiology·M R RekikM A Elazhary
Sep 1, 1987·Journal of Comparative Pathology·B M AdairE R McKillop
Dec 1, 1982·Virology·G E Wilcox, R W Compans
Jun 1, 1995·The Journal of General Virology·M R ShapouriA Silim
Jun 1, 1994·Journal of Virological Methods·W Y WuH K Shieh
Mar 1, 1995·Avian Pathology : Journal of the W.V.P.A·J MeangerG E Wilcox
Jul 1, 1990·Avian Pathology : Journal of the W.V.P.A·L B Endo-Munoz

❮ Previous
Next ❯

Citations

Apr 28, 2000·Journal of Virological Methods·H J LiuC F Lu
Jan 8, 2013·Virology Journal·Ymene Hellal KortAbdeljelil Ghram
Jul 31, 2004·Avian Diseases·Y VassermanJ Pitcovskiad
Dec 29, 2004·Journal of Virological Methods·Silke BruhnHans-Peter Ottiger
Jul 19, 2011·Journal of Virological Methods·Kejun GuoJoseph J Giambrone
Jul 19, 2011·Journal of Virological Methods·Dana GoldenbergJacob Pitcovski
Aug 9, 2019·Avian Pathology : Journal of the W.V.P.A·Seyed Abed MirbagheriArash Ghalyanchilangeroudi
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Awad A ShehataHafez M Hafez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.