Molecular characterization of berberine bridge enzyme genes from opium poppy

Plant Physiology
Peter J FacchiniD Bull

Abstract

In Papaver somniferum (opium poppy) and related species, (S)-reticuline serves as a branch-point intermediate in the biosynthesis of numerous isoquinoline alkaloids. The berberine bridge enzyme (BBE) ([S]-reticuline:oxygen oxidoreductase [methylene bridge forming], EC 1.5.3.9) catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine and represents the first committed step in the pathway leading to the antimicrobial alkaloid sanguinarine. Three unique genomic clones (bbe1, bbe2, and bbe3) similar to a BBE cDNA from Eschscholtzia californica (California poppy) were isolated from opium poppy. Two clones (bbe2 and bbe3) contained frame-shift mutations of which bbe2 was identified as a putative, nonexpressed pseudogene by RNA blot hybridization using a gene-specific probe and by the lack of transient expression of a chimeric gene fusion between the bbe2 5' flanking region and a beta-glucuronidase reporter gene. Similarly, bbe1 was shown to be expressed in opium poppy plants and cultured cells. Genomic DNA blot-hybridization data were consistent with a limited number of bbe homologs. RNA blot hybridization showed that bbe genes are expressed in roots and stems...Continue Reading

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Citations

Jul 5, 2007·Plant Cell Reports·Sonia Gómez-GaleraPaul Christou
Apr 25, 2012·Plant Molecular Biology·Isabel Desgagné-PenixPeter J Facchini
Mar 9, 2004·Trends in Plant Science·Peter J FacchiniBenoit St-Pierre
May 4, 2001·Trends in Plant Science·J MemelinkJ W Kijne
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·W M Chou, T M Kutchan
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Manuella CatterouRajbir S Sangwan
Nov 9, 2002·European Journal of Biochemistry·Takashi MorishigeFumihiko Sato
Feb 7, 2013·Plant & Cell Physiology·Jillian M Hagel, Peter J Facchini
Apr 27, 2012·Plant Physiology·Thu-Thuy T Dang, Peter J Facchini
Feb 5, 1998·Plant Physiology·K D RichardsR C Gardner
Oct 10, 2013·The Plant Cell·Akpevwe OnoyovwePeter J Facchini
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Peter J Facchini
Feb 7, 2008·Annual Review of Plant Biology·Jörg Ziegler, Peter J Facchini
Mar 29, 2014·Planta·Guillaume A W Beaudoin, Peter J Facchini
Mar 24, 2009·Journal of Molecular Biology·Ana SauríIsmael Mingarro
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Ben M AbellMaurice M Moloney
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Peter J Facchini, Vincenzo De Luca
Jul 4, 2006·The Plant Journal : for Cell and Molecular Biology·Nailish SamananiPeter J Facchini
Jan 15, 2013·Biochemical and Biophysical Research Communications·Guillaume A W Beaudoin, Peter J Facchini
Jan 15, 2015·Phytochemistry·Xue ChenPeter J Facchini
Apr 24, 2015·PloS One·Elena FossatiVincent J J Martin
Dec 13, 2016·Chemical Reviews·Man-Cheng TangYi Tang
Feb 16, 2017·Frontiers in Plant Science·Karen M FrickRhonda C Foley
Feb 6, 2018·The Plant Journal : for Cell and Molecular Biology·Manuel BenedettiFelice Cervone

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