Molecular characterization of bmyC gene of the mosquito pupicidal bacteria, Bacillus amyloliquefaciens (VCRC B483) and in silico analysis of bacillomycin D synthetase C protein

World Journal of Microbiology & Biotechnology
Mathivanan AshokkumarA M Manonmani

Abstract

A strain of Bacillus amyloliquefaciens (VCRC B483) exhibiting mosquito pupicidal, keratinase and antimicrobial activities was isolated from mangrove forest ecosystem of Andaman and Nicobar Islands. Molecular characterization of the strain showed the presence of lipopeptide encoding bmyC gene. Phylogenetic tree based on protein sequence of this gene exhibited homology with mycosubtilin synthetase of Bacilus atropheus and Iturin synthetase of Bacillus subtilis and B. amyloliquefaciens. This is the first report on the evolutionary conservation of amino acids concerned with the function and structure of bmyC protein of B. amyloliquefaciens. The presence of valine at the 1197th position in our strain was found to be unique and different from the existing strains of B. subtilis and B. amyloliquefaciens. Molecular modelling studies revealed significant changes in the structure of epimerization domain of the bmyC protein with A1197V variation. Crude metabolite of this strain exhibited antifungal activity against Fusarium sp. and Carvularia sp.

References

Aug 1, 1981·European Journal of Biochemistry·F PeypouxL Delcambe
Oct 1, 1980·The Journal of Antibiotics·F PeypouxL Delcambe
Oct 19, 2004·Annual Review of Microbiology·Robert Finking, Mohamed A Marahiel
Feb 22, 2008·Trends in Microbiology·Marc Ongena, Philippe Jacques
Feb 16, 2010·Current Opinion in Structural Biology·Matthias StriekerMohamed A Marahiel
Jan 6, 2011·FEMS Microbiology Letters·Olfa TabbeneFerid Limam
Jan 12, 2011·The Journal of Microbiology·Lisianne Brittes BenitezAdriano Brandelli
Nov 20, 2012·Applied and Environmental Microbiology·Zhihui XuRuifu Zhang
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 30, 2015·Canadian Journal of Microbiology·Chandra Datta SumiYoung Tae Hahm
Jul 1, 2015·Applied Biochemistry and Biotechnology·Yuzhu HanQingmao Shang

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Datasets Mentioned

BETA
KJ169570
JN801140

Methods Mentioned

BETA
PCR

Software Mentioned

Consurf
align
Discovery Studio
nBlast
Bioedit
CHARMm
Modeller
consurf webserver
MEGA6
bmyC

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