Molecular characterization of Brazilian avian pneumovirus isolates using reverse transcription-polymerase chain reaction, restriction endonuclease analysis and sequencing of a G gene fragment

Avian Pathology : Journal of the W.V.P.A
M A DaniE L Durigon

Abstract

A reverse transcriptase-polymerase chain reaction (RT-PCR) assay was employed to amplify a G gene transcript of the avian pneumovirus (APV) in two European turkey isolates and two Brazilian chicken isolates. The PCR products were digested using restriction endonucleases and the restriction patterns were compared with earlier reported isolate patterns. The same PCR products were cloned into pUC18 and sequenced. Restriction patterns and sequence analyses of the approximately 600 bp products from both Brazilian isolates revealed 99% identity with earlier reported subgroup A APV sequence, suggesting that these isolates belong to this subgroup.

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Citations

Nov 15, 2001·Animal Health Research Reviews·B S Seal
Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T P Berg
Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T C CardosoC W Arns
Sep 30, 2005·Avian Pathology : Journal of the W.V.P.A·Caroline Banet-NoachShimon Perk
Sep 28, 2007·Avian Pathology : Journal of the W.V.P.A·Jorge Luis ChacónAntonio J Piantino Ferreira
Sep 14, 2000·The Veterinary Journal·J K Cook

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