Molecular characterization of carbapenem-resistant Gram-negative bacilli clinical isolates in Algeria

Infection and Drug Resistance
Nadjette BourafaJean-Marc Rolain

Abstract

The aims of this study were to investigate the occurrence of carbapenem-resistant Gram-negative bacilli (GNB) isolated from inpatients and outpatients in Algeria between July and September 2015, and to screen their resistance mechanisms and genetic relatedness. A total of 68 non-redundant isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) antibiotic susceptibility testing was performed using disk diffusion and Etest methods. Carbapenemase activity was carried out using modified Carba NP test, EDTA assay, and the modified Hodge test. Molecular characterization of carbapenemases and extended-spectrum β-lactamase (ESBL) genes were detected by standard PCR and sequencing. Genotyping of carbapenem-resistant isolates was performed by multilocus sequence typing (MLST) analysis. Of the 68 GNB isolates, 13 (19%) showed reduced susceptibility to carbapenems, including, four Klebsiella pneumoniae, one Escherichia coli, six Acinetobacter baumannii, and two Pseudomonas aeruginosa. blaOXA-48 gene was detected in the five Enterobacteriaceae isolates, and blaOXA-23 was identified in all A. baumannii isolates. OprD mutations were revealed in the two P. aeruginosa isolates. A total of 11 out of ...Continue Reading

Citations

Oct 29, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Abdelaziz Touati, Assia Mairi
Mar 26, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nadia ChaalalAlix Pantel
Dec 4, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Larbi Zakaria NabtiJean-Marc Rolain

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Methods Mentioned

BETA
PCR
Genotyping

Software Mentioned

Codon Code Aligner
biotyper

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