Molecular characterization of CcpA and involvement of this protein in transcriptional regulation of lactate dehydrogenase and pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis

Applied and Environmental Microbiology
Narito AsanumaTsuneo Hino

Abstract

A ccpA gene that encodes global catabolite control protein A (CcpA) in Streptococcus bovis was identified and characterized, and the involvement of CcpA in transcriptional control of a gene (ldh) encoding lactate dehydrogenase (LDH) and a gene (pfl) encoding pyruvate formate-lyase (PFL) was examined. The ccpA gene was shown to be transcribed as a monocistronic operon. A catabolite-responsive element (cre) was found in the promoter region of ccpA, suggesting that ccpA transcription in S. bovis is autogenously regulated. CcpA required HPr that was phosphorylated at the serine residue at position 46 (HPr-[Ser-P]) for binding to the cre site, but glucose 6-phosphate, fructose 1,6-bisphosphate, and NADP had no effect on binding. Diauxic growth was observed when S. bovis was grown in a medium containing glucose and lactose, but it disappeared when ccpA was disrupted, which indicates that CcpA is involved in catabolite repression in S. bovis. The level of ccpA mRNA was higher when cells were grown on glucose than when they were grown on lactose, which was in line with the level of ldh mRNA. When cells were grown on glucose, the ldh mRNA level was lower but the pfl mRNA level was higher in a ccpA-disrupted mutant than in the parent str...Continue Reading

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Dec 27, 2002·The Journal of General and Applied Microbiology·Narito AsanumaTsuneo Hino

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Citations

Nov 6, 2007·Nature Reviews. Microbiology·Abraham L Sonenshein
Dec 8, 2005·Applied and Environmental Microbiology·Sylviane DerzelleFrançoise Rul
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Jun 2, 2010·The Journal of General and Applied Microbiology·Narito AsanumaTsuneo Hino
Feb 14, 2012·International Journal of Food Microbiology·Teresa ZottaEugenio Parente
Feb 1, 2011·Journal of Biotechnology·Haruhiko TeramotoHideaki Yukawa
Apr 25, 2007·Critical Reviews in Microbiology·Magdalena Kowalczyk, Jacek Bardowski
Aug 3, 2016·Infection and Immunity·Krystle A BlanchetteCarlos J Orihuela
Jul 31, 2016·Applied and Environmental Microbiology·Lianmin ChenMengzhi Wang
Feb 3, 2021·Journal of Bacteriology·Anne TroitzschJan Pané-Farré
Jul 18, 2015·Microbiology Spectrum·Anthony R RichardsonAbraham L Sonenshein

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