Molecular characterization of CD9 and CD63, two tetraspanin family members expressed in trout B lymphocytes

Developmental and Comparative Immunology
Rosario CastroCarolina Tafalla

Abstract

Tetraspanins are a family of membrane-organizing proteins, characterized by the presence of four highly conserved transmembrane regions that mediate diverse physiological functions. In the current study, we have identified two novel tetraspanin members in rainbow trout (Oncorhynchus mykiss), homologs to mammalian CD9 and CD63. Both genes were expressed in muscle, skin, gills, hindgut, gonad, liver, spleen, head kidney, thymus and peripheral blood leukocytes. Throughout the early life cycle stages, CD9 mRNA levels significantly increased after first feeding, whereas CD63 transcription remained constant during all the developmental stages analyzed. In response to an experimental bath infection with viral hemorrhagic septicemia virus (VHSV), CD9 transcription was down-regulated in the gills, while CD63 mRNA levels were down-regulated in the head kidney. Instead, when the virus was intraperitoneally injected, the transcription of both genes was significantly up-regulated in peritoneal cells at several days post-infection. Additionally, both genes were transcriptionally up-regulated in the muscle of trout injected with a VHSV DNA vaccine. To gain insight on the relation of these tetraspanins with B cell activity we determined their ...Continue Reading

References

May 1, 1991·The Journal of Experimental Medicine·P J PetersH J Geuze
Dec 1, 1994·Immunology Today·M D Wright, M G Tomlinson
Oct 25, 2006·The Journal of Experimental Medicine·Seon-ah HaSidonia Fagarasan
Mar 25, 2011·Biochemical Society Transactions·Eleanor Livingston JonesMark D Wright
Mar 25, 2011·Biochemical Society Transactions·Annemiek B van Spriel
Mar 25, 2011·Biochemical Society Transactions·Hong-Xing WangMartin E Hemler
Jun 1, 2011·PloS One·Michael Z TrikićLynda J Partridge
Jun 11, 2011·European Journal of Immunology·Sven H PetersenFedor Berditchevski
Jan 8, 2013·Biochemical and Biophysical Research Communications·Sun-Ok YoonYong Sung Choi
Aug 6, 2013·Fish & Shellfish Immunology·Tomáš KorytářBernd Köllner
Oct 18, 2013·International Journal of Molecular Sciences·Bharat Bhusan PatnaikYeon Soo Han
Nov 21, 2014·Journal of Virology·Beatriz AbósCarolina Tafalla

❮ Previous
Next ❯

Citations

Jun 6, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Beatriz AbósCarolina Tafalla
Nov 22, 2016·Journal of Immunology Research·Mingjia YuQiang Xia
Jun 8, 2018·Frontiers in Immunology·Maria Laura SaizFrancisco Sánchez-Madrid
Aug 4, 2018·Frontiers in Immunology·Fagui ZouJianbo Sun
Feb 9, 2021·Fish & Shellfish Immunology·Sumi JungJehee Lee
Apr 17, 2021·Developmental and Comparative Immunology·Z H FanX Z Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.