Molecular characterization of clinical Saccharomyces cerevisiae isolates and their association with non-clinical strains

Systematic and Applied Microbiology
R de LlanosM Teresa Fernández-Espinar

Abstract

We assessed the molecular characterization of 96 clinical isolates of S. cerevisiae from a Spanish medical institution and we compared them with 6 non-clinical strains isolated from wine, beer and bread and 1 S. boulardii strain collected from a commercial preparation. The strains were subjected to HinfI mtDNA restriction analysis and PCR amplification of delta sequences. Although both techniques are appropriate for routine clinical analysis, that based on PCR turned out to be the most discriminating. This study, apart from providing tools for clinical application, deals with the relationships between clinical and non-clinical strains. The two baker's yeasts analysed shared mtDNA and PCR patterns with a group of 31 clinical isolates. An exogenous entry of a strain was also reflected in the case of 19 clinical isolates and the therapeutic strain S. boulardii. Both baker's yeasts and S. boulardii were identified respectively among 32.3% and 19.8% of the clinical isolates and there seemed to be a connection between their ability to colonize humans and their ability to cause vaginal infection. The rest of food isolates were not grouped with clinical strains.

Citations

Jun 1, 2014·PloS One·Silvia LlopisMaría T Fernández-Espinar
Jan 19, 2016·Frontiers in Microbiology·Roberto Pérez-Torrado, Amparo Querol
May 23, 2012·International Journal of Food Microbiology·Roberto Pérez-TorradoAmparo Querol
Sep 18, 2010·Diagnostic Microbiology and Infectious Disease·Karl V ClemonsDavid A Stevens
Mar 27, 2010·Journal of Applied Microbiology·N R BüchlS Scherer
Sep 1, 2014·Journal of Proteomics·Carolina Hernández-HaroConcha Gil
Jun 20, 2006·International Journal of Food Microbiology·Rosa de LlanosMaría Teresa Fernández-Espinar
Nov 18, 2010·International Journal of Food Microbiology·R de LlanosM T Fernández-Espinar
Apr 28, 2011·FEMS Microbiology Reviews·Stefan Brückner, Hans-Ulrich Mösch
Jul 22, 2017·Molecular Nutrition & Food Research·Walter P PflieglerIstván Pócsi
Jul 12, 2005·Yeast
Jun 19, 2016·G3 : Genes - Genomes - Genetics·Yuan O ZhuDmitri A Petrov
Aug 20, 2019·Microbiological Research·Alexandra ImreWalter P Pfliegler

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