Molecular Characterization of Clostridium difficil e Isolates in China From 2010 to 2015

Frontiers in Microbiology
Xiao-Shu LiuJin-Xing Lu

Abstract

Clostridium difficile infection (CDI) has become a worldwide public health problem causing high mortality and a large disease burden. Molecular typing and analysis is important for surveillance and infection control of CDI. However, molecular characterization of C. difficile across China is extremely rare. Here, we report on the toxin profiles, molecular subtyping with multilocus sequence typing (MLST) and PCR ribotyping, and epidemiological characteristics of 199 C. difficile isolates collected between 2010 through 2015 from 13 participating centers across China. We identified 35 STs and 27 ribotypes (RTs) among the 199 C. difficile isolates: ST35 (15.58%), ST3 (15.08%), ST37 (12.06%), and RT017 (14.07%), RT001 (12.06%), RT012 (11.56%) are the most prevalent. One isolate with ST1 and 8 isolates with ST 11 were identified. We identified a new ST in this study, denoted ST332. The toxin profile tcdA+tcdB+tcdC+tcdR+tcdE+CDT- (65.83%) was the predominant profile. Furthermore, 11 isolates with positive binary toxin genes were discovered. According to the PCR ribotyping, one isolate with RT 027, and 6 isolates with RT 078 were confirmed. The epidemiological characteristics of C. difficile in China shows geographical differences, and ...Continue Reading

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Citations

Aug 15, 2019·Critical Reviews in Clinical Laboratory Sciences·Jingpeng Yang, Hong Yang
Aug 24, 2019·Frontiers in Cellular and Infection Microbiology·Jingpeng Yang, Hong Yang
Nov 21, 2020·Diagnostic Microbiology and Infectious Disease·Colin D LloydLinda Chui
Jan 12, 2022·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Charles DarkohHerbert L DuPont

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Methods Mentioned

BETA
PCR
electrophoresis
ribotyping

Software Mentioned

QIAxcel ScreenGel
BioNumerics
DNAStar
MEGA7

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