Molecular Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus Isolated from Skin and Pus Samples of Outpatients in Japan

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Tetsuo YamaguchiTetsuya Matsumoto

Abstract

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is now endemic in the United States. In Japan, CA-MRSA infections and CA-MRSA surveillance have been scarcely reported. In this study, we conducted a nationwide survey of CA-MRSA in Japan. We collected MRSA strains isolated from outpatients with skin and soft-tissue infection (SSTI) at 107 medical facilities from 24 prefectures in 2010 and 2012. Among 10,385 clinical samples from SSTI patients, 3,581 S. aureus isolates (35%) were obtained and 673 of the S. aureus strains (19%) were identified as MRSA. Among 625 MRSA strains tested in this study, 266 strains (43%) and 114 strains (18%) were classified as SCCmec types IV and V, respectively. Detection of virulence genes was as follows: Panton-Valentine leukocidin (PVL) gene (57 strains, 9%), exfoliative toxin (ET) gene (179 strains, 29%), toxic shock syndrome toxin-1 (TSST-1) gene (195 strains, 31%), or none. PVL-positive strains were classified into eight sequence types (STs) (i.e., ST1, ST5, ST8, ST22, ST30, ST452, ST59, and ST154) and six clonal complexes (i.e., CC1, CC5, CC8, CC22, CC30, and CC59). Only 10 PVL-positive strains (2%) were pulsed-field type USA300 clone. There were a wide variety of CA-MR...Continue Reading

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Citations

Jun 25, 2017·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Shinichi WatanabeTakuro Kanekura
Apr 21, 2018·Journal of Medical Microbiology·Hidemasa NakaminamiNorihisa Noguchi
Jul 28, 2017·Therapeutic Advances in Infectious Disease·Haruko MiyazakiTetsuya Matsumoto
Sep 21, 2016·Expert Review of Anti-infective Therapy·Kyungmin Huh, Doo Ryeon Chung
Jul 20, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Kavitha Shettigar, Thokur Sreepathy Murali

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MLST

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