Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in children in Egypt

Parasites & Vectors
Doaa NaguibLihua Xiao

Abstract

The transmission of Cryptosporidium spp. and Giardia duodenalis into humans varies according to species/genotypes of the pathogens. Although infections with both parasites are recorded in Egypt, few data are available on the distribution of Cryptosporidium species and G. duodenalis genotypes. The present study assessed the occurrence and genetic diversity of Cryptosporidium spp. and G. duodenalis in Egyptian children. In the present study, 585 fecal specimens were collected from children eight years old and younger in three provinces (El-Dakahlia, El-Gharbia and Damietta) during March 2015 to April 2016. PCR-RFLP analysis of the small subunit rRNA gene and sequence analysis of the 60 kDa glycoprotein gene were used to detect and subtype Cryptosporidium spp., respectively, whereas PCR and sequence analyses of the triose phosphate isomerase, glutamate dehydrogenase and β-giardin genes were used to detect and genotype Giardia duodenalis. The overall infection rates of Cryptosporidium spp. and G. duodenalis were 1.4% and 11.3%, respectively. The Cryptosporidium species identified included C. hominis and C. parvum, each with three subtype families. The C. hominis subtypes were IbA6G3 (n = 2), IdA17 (n = 1), IdA24 (n = 1) and IfA14G1...Continue Reading

References

Jul 31, 1999·Applied and Environmental Microbiology·L XiaoA A Lal
Jun 7, 2003·Journal of Clinical Microbiology·Margarida AlvesFrancisco Antunes
Aug 22, 2003·Annals of Tropical Medicine and Parasitology·F A Al-BraikenC A Hart
Jan 14, 2004·Emerging Infectious Diseases·Irshad M SulaimanLihua Xiao
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Carolyn M ReadR C Andrew Thompson
Apr 16, 2005·Journal of Tropical Pediatrics·Ibrahim Adib Abdel-MessihRobert Frenck
Jun 16, 2005·Journal of Clinical Microbiology·Irshad M SulaimanLihua Xiao
May 6, 2006·Diagnostic Microbiology and Infectious Disease·Hanon El-MohamadyRobert W Frenck
Apr 11, 2009·Experimental Parasitology·Lihua Xiao
Jan 30, 2010·Experimental Parasitology·Nawal HijjawiUna Ryan
Jan 15, 2011·Journal of Medical Microbiology·Jamshaid IqbalParsotam Ravjee Hira
Jan 15, 2011·Clinical Microbiology Reviews·Yaoyu Feng, Lihua Xiao
May 25, 2011·Parasitology Research·Nour M Abd El KaderIsabel de Fuentes
Feb 7, 2012·Emerging Infectious Diseases·Yaoyu FengLihua Xiao
Mar 28, 2012·The Korean Journal of Parasitology·Ekhlas H Abdel-HafeezFadia A Moslam
Feb 2, 2013·Parasitology·N A AlyousefiR Mahmud
Jul 17, 2013·International Journal for Parasitology·Una Ryan, Simone M Cacciò
Oct 4, 2014·The Lancet Infectious Diseases·William CheckleyEric R Houpt
Mar 10, 2015·PLoS Neglected Tropical Diseases·Daniel EibachJürgen May
Oct 7, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A A El-BadryE Y Abu-Sarea
Jun 2, 2016·The Onderstepoort Journal of Veterinary Research·Nada Abu SamraPeter N Thompson
Jul 28, 2016·Parasitology Research·Mousa A M IsmailAyman A El-Badry
Nov 5, 2016·Current Opinion in Microbiology·Elin EinarssonStaffan G Svärd
Mar 13, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Tao WangRobin B Gasser
Apr 22, 2017·Parasites & Vectors·Sylvia Afriyie Squire, Una Ryan
Sep 29, 2017·Food and Waterborne Parasitology·Lihua Xiao, Yaoyu Feng

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Citations

Sep 4, 2019·PloS One·Tamar GrossmanLea Valinsky
Feb 23, 2019·Parasitology Research·I HolzhausenA Daugschies
Mar 4, 2020·Acta Parasitologica·Naglaa Fathi Abd El-LatifAhmed Hamdi Mohamed Abd El-Malek
Jan 7, 2021·Transboundary and Emerging Diseases·Zhenjie ZhangYaoyu Feng
Jan 26, 2021·Parasite Epidemiology and Control·Hanako IwashitaTetsu Yamashiro
Feb 26, 2021·Clinical Microbiology Reviews·Xin YangYaoyu Feng
May 22, 2021·Frontiers in Cellular and Infection Microbiology·Marwa ChourabiMarawan Abu-Madi
Aug 14, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Barbara BandaPaul Kelly
Sep 4, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Heba ElhadadHend A El-Taweel
Sep 4, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Samira Metwally MohammadMarwa A Salama

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Datasets Mentioned

BETA
AB781127
KY696816
AB480877

Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

ChromasPro
SPSS
ClustalX

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