PMID: 12751316May 20, 2003Paper

Molecular characterization of dengue viruses type 1 and 2 isolated from a concurrent human infection

Revista Do Instituto De Medicina Tropical De São Paulo
Cecília Luiza Simões dos SantosI M Rocco

Abstract

In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively.

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Citations

Aug 13, 2013·PloS One·Camila Malta RomanoEsper Georges Kallas
Jul 1, 2014·PloS One·Valquiria do Carmo Alves MartinsMaria Paula Gomes Mourão
Dec 3, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Lieselotte CnopsMarjan Van Esbroeck
Oct 9, 2016·BMC Infectious Diseases·Angela Ferreira Lopes de Teive E ArgoloCelina Maria Turchi Martelli
Jul 5, 2012·Revista Da Sociedade Brasileira De Medicina Tropical·Ana Caroline Dalla BonaMário Antônio Navarro-Silva
Feb 13, 2007·Memórias do Instituto Oswaldo Cruz·Fernanda Montenegro de Carvalho AraújoIvo Castelo Branco Coelho
Aug 3, 2004·Revista Do Instituto De Medicina Tropical De São Paulo·Cecília Luiza S SantosIray Maria Rocco
Jan 6, 2018·Journal of Global Infectious Diseases·Francisco Espinoza-GómezUriel A López-Lemus
Nov 8, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mânlio Tasso de Oliveira MotaMaurício Lacerda Nogueira
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Sep 23, 2016·PLoS Neglected Tropical Diseases·Mohd Jaseem KhanVictor Hugo Aquino
Mar 16, 2019·BMC Research Notes·Sri MasyeniR Tedjo Sasmono

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