Molecular characterization of diarrheagenic Escherichia coli from Libya.

The American Journal of Tropical Medicine and Hygiene
Mostafa Mohamed M AliKhalifa Sifaw Ghenghesh

Abstract

Diarrheagenic Escherichia coli (DEC) are important enteric pathogens that cause a wide variety of gastrointestinal diseases, particularly in children. Escherichia coli isolates cultured from 243 diarrheal stool samples obtained from Libyan children and 50 water samples were screened by polymerase chain reaction (PCR) for genes characteristic of enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), and enteroinvasive E. coli (EIEC). The DEC were detected in 21 (8.6%) children with diarrhea; 10 (4.1%) cases were identified as EAEC, 3 (1.2%) as EPEC, and 8 (3.3%) were ETEC; EHEC, and EIEC were not detected. All DEC were grouped phylogenetically by PCR with the majority (> 70%) identified as phylogenetic groups A and B1. The EAEC isolates were also tested for eight genes associated with virulence using PCR. Multi-virulence (≥ 3 virulence factors) was found in 50% of EAEC isolates. Isolated EAEC possessed different virulence traits and belonged to different phylogenetic groups indicating their heterogeneity.

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Citations

Apr 26, 2014·The Libyan Journal of Medicine·Mohamed O Ahmed, Yousef M Abouzeed
Apr 15, 2016·Paediatrics and International Child Health·Eamonn TrainorNigel Cunliffe
May 6, 2015·Paediatrics and International Child Health·Eamonn TrainorNigel Cunliffe
Dec 13, 2019·Frontiers in Public Health·Habeeb Kayode SakaJulio Alvarez
Nov 8, 2017·Journal of Epidemiology and Global Health·P Ramya RaghavanAttayur Purushothaman Sugunan
Feb 21, 2019·Arquivos De Gastroenterologia·Roger Lafontaine Mesquita TabordaNajla Benevides Matos
May 30, 2019·BMC Research Notes·Cristian Pérez-Corrales, Kevin Leandro-Sandí

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