Molecular characterization of Dirofilaria spp. circulating in Portugal

Parasites & Vectors
Cátia C FerreiraSilvana Belo

Abstract

Dirofilariosis is a potentially zoonotic parasitic disease, mainly transmitted by mosquito vectors in many parts of the world. Data concerning the canine Dirofilaria species currently circulating in Portugal is scarce. Thereby, a large-scale study was conducted to determine the Dirofilaria spp. present in Portugal, based on a molecular approach, and also to optimize a reliable and highly sensitive species-specific polymerase chain reaction (PCR) assay that could be used for the simultaneous detection and differentiation of Dirofilaria immitis, Dirofilaria repens, and other concurrent filarial species in animal reservoirs. Blood samples were collected from three districts of Portugal (Coimbra, Santarém and Setúbal) between 2011 and 2013. Samples were tested using rapid immunomigration tests (Witness® Dirofilaria), modified Knott's technique and acid phosphatase histochemical staining. In addition, molecular analysis was performed by amplification of the internal transcribed spacer (ITS) region using two different PCR protocols, specific for molecular screening of canine filarial species. Of the 878 dogs sampled, 8.8% (n = 77) were positive for D. immitis circulating antigen and 13.1% (n = 115) positive for microfilariae by the m...Continue Reading

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Citations

Dec 19, 2019·Veterinary World·Djamel TahirPhilippe Parola
Dec 4, 2019·Epidemiology and Infection·A P Fontes-SousaJ A Montoya-Alonso
Jun 27, 2020·Acta Parasitologica·Mehmet Fatih AydınNazir Dumanlı
May 6, 2021·Medical and Veterinary Entomology·R R S ManojD Otranto
May 25, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Sandra NoackPaul M Selzer

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Datasets Mentioned

BETA
AY621480.1
AY621481.1
EU087699
EU182329
FJ263464.1
FJ2634571
HM126606.1

Methods Mentioned

BETA
PCR
blood collection
electrophoresis

Software Mentioned

PHASE
BioEdit
SPSS
MEGA
BLAST

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