PMID: 20646459Jul 22, 2010Paper

Molecular characterization of drug-resistant and extensive-drug resistant isolates of Mycobacterium tuberculosis in Shenzhen of China

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
Jing GuiLi Zhang

Abstract

To study the molecular characterization of drug-resistant and extensive-drug resistant isolates of Mycobacterium tuberculosis (MTB) in Shenzhen of China during 2007 - 2008. According to the standards of WHO and International Union Against Tuberculosis and Lung Disease (IUATLD), 136 strains of MTB were collected by performing drug sensitivity test (DST) to isoniazid, rifampicin, streptomycin, ofloxacin and kanamycin on Lowenstein-Jensen in 1% proportion method. Genetic mutations in the corresponding resistance genes (rpoB, katG, rpsL, rrs((1)), gyrA/B, rrs((2))) in these MTB isolates were identified by PCR, followed by DNA sequencing of the purified PCR products. A total of 123 isolates were collected. Seventy-three isolates were drug resistant, and 50 isolates were drug susceptible. Among the isolates that were resistant to isoniazid, rifampicin, streptomycin, ofloxacin and kanamycin, the proportion of isolates that harboured mutations in the respective genes was 44/52, 44/47, 28/41, 11/11, and 11/18, respectively. For katG gene, the mutation detected was S315T or S315N. For rpoB, the most frequently found changes were S531L (30/44) and H526D (9/44) or H526R (1/44). For the reported mutations related with streptomycin-resistant...Continue Reading

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