Molecular characterization of Echinococcus granulosus sensu lato from farm animals in Egypt

PloS One
Said AmerLihua Xiao

Abstract

Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.

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Dec 22, 2019·Parasitology Research·Christian HidalgoCristian A Alvarez Rojas
Jul 27, 2018·BMC Veterinary Research·Molouk BeiromvandSharif Maraghi
Aug 11, 2019·Parasites & Vectors·Yunliang ShiYichao Yang
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Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Reem M RamadanMohamed M El-Bahy

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Datasets Mentioned

BETA
AB921019
AB921053
AB921054
AB921090
AB921091
AB921125
AB921068
AB921105
AB921075
AB921111

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MrBayes
ClustalX
TCS
Mesquite
ChromasPro

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