Molecular Characterization of Escherichia coli Isolated from Chickens with Colibacillosis in Senegal

Foodborne Pathogens and Disease
Passoret VounbaRianatou Bada Alambédji

Abstract

Avian pathogenic Escherichia coli (APEC), a subset of extraintestinal pathogenic E. coli (ExPEC), are the etiologic agent of avian colibacillosis, one of the main causes of economic losses in the poultry industry. The aim of this study was to characterize E. coli isolated from diseased chickens in Senegal to elucidate their virulence potential and antimicrobial resistance (AMR). A total of 58 isolates, each from a separate farm, were characterized for AMR, virulence, and AMR genes, phylogroup, serogroup, biofilm formation, and pulsed-field gel electrophoresis, and for two isolates, whole-genome sequencing (WGS). Fifty isolates (86.2%) were multidrug resistant. Many AMR genes were detected, including variants of blaCTX-M encoding resistance to third-generation cephalosporins (five isolates [8.6%]). Most fluoroquinolone-nonsusceptible isolates (21/26) were carriers of mutations in gyrA (Ser83Leu, Asp87Asn, and/or Asp87Tyr) and/or parC (Ser80Ile) genes. Forty-nine (84.5%) isolates exhibited at least one of the virulence markers of APEC, among which 23 (39.7%) were defined as potential virulent APEC. In addition, 10 isolates, of which 9 were defined as APEC, carried virulence profiles corresponding to ExPEC. Seven isolates, of whic...Continue Reading

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Citations

May 1, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Passoret VounbaJohn Morris Fairbrother
Dec 1, 2020·Microbial Genomics·Ebenezer Foster-NyarkoMark John Pallen

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Illumina BaseSpace Sequence Hub
BioNumerics ( Applied Maths )
Bacterial Analysis Pipeline Tool ( GoSeqIt
Molecular Evolutionary Genetics Analysis

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