Molecular characterization of fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates from Shanghai, China

Diagnostic Microbiology and Infectious Disease
Cuiyun ZhuWing-Cheong Yam

Abstract

China is one of the countries with the highest prevalence of fluoroquinolone-resistant (FQ(r)) Mycobacterium tuberculosis. Nevertheless, knowledge on the molecular characterization of the FQ(r)M. tuberculosis strains of this region remains very limited. This study was performed to investigate the frequencies and types of mutations present in FQ(r)M. tuberculosis clinical isolates collected in Shanghai, China. A total of 206 FQ(r)M. tuberculosis strains and 21 ofloxacin-sensitive (FQ(s)) M. tuberculosis strains were isolated from patients with pulmonary tuberculosis in Shanghai. The phenotypic drug susceptibilities were determined by the proportion method, and the mutations inside quinolone resistance-determining region (QRDR) of gyrA and gyrB genes were identified by DNA sequence analyses. Among 206 FQ(r)M. tuberculosis strains, 44% (90/206) were multidrug-resistant isolates and 39% (81/206) were extensively drug-resistant isolates. Only 9% (19/206) were monoresistant to ofloxacin. In total, 79.1% (163/206) of FQ(r) isolates harboured mutations in either gyrA or gyrB QRDR. Mutations in gyrA QRDR were found in 75.7% (156/206) of FQ(r) clinical isolates. Among those gyrA mutants, a majority (75.6%) harboured mutations at amino ac...Continue Reading

References

Apr 19, 2003·The Journal of Antimicrobial Chemotherapy·Richard Wise
Jul 3, 2003·The Lancet Infectious Diseases·Amy Sarah GinsburgWilliam R Bishai
Oct 6, 2005·The Journal of Antimicrobial Chemotherapy·Tsi-Shu HuangYung-Ching Liu
Mar 16, 2007·The Journal of Antimicrobial Chemotherapy·Raphael C Y ChanAugustine F B Cheng
Jun 19, 2008·Antimicrobial Agents and Chemotherapy·Igor MokrousovNalin Rastogi
Apr 15, 2009·Antimicrobial Agents and Chemotherapy·Peng XuQian Gao
Apr 23, 2011·American Journal of Respiratory and Critical Care Medicine·Gail E LouwThomas C Victor

❮ Previous
Next ❯

Citations

Apr 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abdul NaeemKhalid Khan
Dec 24, 2014·Antimicrobial Agents and Chemotherapy·Christine BernardAlexandra Aubry
Oct 30, 2013·Antimicrobial Agents and Chemotherapy·Zhijian ZhangYanlin Zhao
Apr 16, 2014·Antimicrobial Agents and Chemotherapy·Li-li ZhaoKang-lin Wan
Apr 30, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fernanda MaruriTimothy R Sterling
May 14, 2014·FEMS Microbiology Letters·Jie LuZhuo Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.