Molecular characterization of foot-and-mouth disease viruses collected from Sudan.

Transboundary and Emerging Diseases
M HabielaD J Paton

Abstract

The aim of this study was to characterize foot-and-mouth disease (FMD) viruses collected between 2004 and 2008 from Sudan, a country where FMD is endemic. Using virus isolation and antigen ELISA, three FMD virus serotypes (O, A and SAT2) were detected in 24 samples that were submitted to the FAO World Reference Laboratory for FMD. Pan-serotypic real-time RT-PCR assays targeting the 5' untranslated region (5'UTR) and 3D genes of FMD virus were also used to contribute to the laboratory diagnosis of these cases. The lack of concordant results between the real-time RT-PCR assays for three serotype O viruses was attributed to four nucleotide mismatches in the 5'UTR PCR primer and probe sites (three substitutions for the sense-primer and one in the TaqMan(®) probe region). Taken together, the laboratory results showed that recent FMD outbreaks that occurred during 2008 in northern and central Sudan were caused by serotypes O and SAT2, while serotype A was last detected in 2006. Phylogenetic analyses of VP1 sequences from these viruses were used to determine the relationships with 23 older viruses from Sudan and other viruses from West and East Africa. For serotype O, closest genetic identities were between concurrent and historical S...Continue Reading

References

May 9, 1998·Journal of Comparative Pathology·R P Kitching
Feb 24, 2001·Annals of the New York Academy of Sciences·M RweyemamuK Wojciechowski
Jun 8, 2002·Journal of the American Veterinary Medical Association·Johnny D CallahanWilliam M Nelson
Jan 16, 2003·Virus Research·N J Knowles, A R Samuel
May 8, 2004·Journal of Clinical Microbiology·B M de C BronsvoortK L Morgan
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Donald P KingTammy R Beckham
Apr 3, 2007·Journal of Virological Methods·Andrew E ShawDonald P King
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 13, 2007·BMC Evolutionary Biology·Alexei J Drummond, Andrew Rambaut
Feb 9, 2008·Emerging Infectious Diseases·Nick J KnowlesDavid J Paton
Apr 10, 2008·Molecular Biology and Evolution·David Posada
May 13, 2009·Transboundary and Emerging Diseases·N J KnowlesD J Paton
Oct 1, 2009·Emerging Infectious Diseases·Gelagay AyeletNick J Knowles
Mar 1, 1983·Revue Scientifique Et Technique·E M E Abu Elzein

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Citations

Jan 21, 2014·Tropical Animal Health and Production·Sabenzia N WekesaKirsten Tjørnehøj
Sep 5, 2014·Tropical Animal Health and Production·Mahmoud Mohey Elhaig, Mohamed Nagi Elsheery
May 23, 2015·Transboundary and Emerging Diseases·B P BritoA M Perez
Nov 5, 2013·The Journal of General Virology·A B LudiJ M Hammond
Jan 28, 2014·Transboundary and Emerging Diseases·C J KasangaD P King
Dec 26, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sabenzia N WekesaHans R Siegismund
Nov 1, 2015·Transboundary and Emerging Diseases·T TekleghiorghisA Dekker
Mar 14, 2014·Clinical and Vaccine Immunology : CVI·Tesfaalem TekleghiorghisAldo Dekker
Jul 24, 2014·Transboundary and Emerging Diseases·T TekleghiorghisA Dekker
Dec 18, 2019·Veterinary Medicine and Science·Arada Izzedine Abdel-AzizLabib Bakkali Kassimi
Dec 28, 2019·Transboundary and Emerging Diseases·D O EhiziboloJ Arzt
Oct 3, 2012·Transboundary and Emerging Diseases·H A AhmedD P King
Jun 24, 2020·Transboundary and Emerging Diseases·Daniel GizawDonald P King
Aug 27, 2021·Scientific Reports·Bashir SalimGuillermo Giovambattista
Sep 4, 2021·Veterinary Research Communications·Wefag AlfouzNussieba A Osman

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