Molecular characterization of GrlA, a specific positive regulator of ler expression in enteropathogenic Escherichia coli.

Journal of Bacteriology
Rafael JiménezJ L Puente

Abstract

Enteropathogenic Escherichia coli (EPEC) infections are characterized by the formation of attaching and effacing (A/E) lesions on the surfaces of infected epithelial cells. The genes required for the formation of A/E lesions are located within the locus of enterocyte effacement (LEE). Ler is the key regulatory factor controlling the expression of LEE genes. Expression of the ler gene is positively regulated by GrlA, which is encoded by the LEE. Here, we analyze the mechanism by which GrlA positively regulates ler expression and show that in the absence of H-NS, GrlA is no longer essential for ler activation, further confirming that GrlA acts in part as an H-NS antagonist on the ler promoter. Single-amino-acid mutants were constructed to test the functional significance of the putative helix-turn-helix (HTH) DNA binding motif found in the N-terminal half of GrlA, as well as at the C-terminal domain of the protein. Several mutations within the HTH motif, but not all, completely abolished GrlA activity, as well as specific binding to its target sequence downstream from position -54 in the ler regulatory region. Some of these mutants, albeit inactive, were still able to interact with the negative regulator GrlR, indicating that los...Continue Reading

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Citations

Oct 5, 2013·Nature Communications·Abhilash PadavannilJ Sivaraman
Feb 5, 2016·Biological Procedures Online·Marisa EganShantanu Bhatt
Sep 8, 2011·Molecular Microbiology·Víctor H BustamanteJosé L Puente
Oct 10, 2012·Molecular Microbiology·Patricia B LodatoJames B Kaper
Aug 2, 2017·Journal of Bacteriology·R Christopher D Furniss, Abigail Clements
Apr 3, 2015·Microbiology and Molecular Biology Reviews : MMBR·Christopher A VakulskasTony Romeo
Nov 8, 2016·Frontiers in Cellular and Infection Microbiology·Meztlli O GaytánBertha González-Pedrajo
Aug 25, 2018·Frontiers in Microbiology·Amy Platenkamp, Jay L Mellies
May 26, 2018·FEMS Microbiology Letters·Biao YuanSpyridoula Karamanou

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