Molecular characterization of hepatitis E virus in patients with acute hepatitis in Venezuela

Journal of Medical Virology
Cristina Gutiérrez GarcíaMaría G Guzmán

Abstract

Hepatitis E virus (HEV) causes a common infection in developing countries. HEV infection occurs as outbreaks, as sporadic clinical cases and as large epidemics in endemic areas. The objective of this study was to determine the presence of HEV infection in patients with clinical suspicion of hepatitis A virus (HAV) infection, referred to the Instituto Nacional de Higiene "Rafael Rangel" in Venezuela. Seventy-four sera were tested for anti-HAV and anti-HEV IgM antibodies. HEV-RNA was amplified from anti-HEV IgM positive sera using nested reverse transcription polymerase chain reaction for ORF1 (RNA dependent RNA polymerase region) and the amplicons sequenced for phylogenetic analysis. The frequency of anti-HEV IgM was 22/74 (30%) in the samples tested. Dual infection with HAV and HEV was found in 31% (12/39) of anti-HAV IgM positive patients. Viremia was detected in 3/22 (14%) of sera positive for anti-HEV IgM. Two HEV strains were classified as genotype 1 and one as genotype 3, which were closely related to Yam 67 (north of India) and US1 isolates from the USA, respectively. These findings suggest that HEV is an important cause of acute viral hepatitis in Venezuela as a single infection or co-infection with HAV, with high morbid...Continue Reading

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Citations

Jan 24, 2013·Virus Research·Santiago MirazoJuan Arbiza
Feb 3, 2016·FEMS Microbiology Letters·Denise de Almeida RamosRafael Frandoloso
Feb 6, 2013·Journal of Medical Virology·Ana Maria PassosCelso Francisco Hernandes Granato
Apr 17, 2013·Journal of Medical Virology·J M EchevarríaL A Rodríguez-Lay
Jan 23, 2014·Enfermedades infecciosas y microbiología clínica·José Manuel EchevarríaAna Avellón
Aug 26, 2014·BMC Infectious Diseases·Claudia Lamarca VitralMarcelo Urbano Ferreira
May 19, 2017·PloS One·Paula A BaezMaria-Cristina Navas
May 23, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·María B PisanoViviana E Ré
Jan 8, 2014·Clinical Microbiology Reviews·Lisa J KrainAlain B Labrique
Jan 8, 2014·Clinical Microbiology Reviews·Nassim KamarJacques Izopet
Aug 29, 2013·Current Opinion in Infectious Diseases·Harry R DaltonRichard P Bendall
Mar 17, 2018·BioMed Research International·Juliana Gil MelgaçoMarcelo Alves Pinto

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